In vitro Cytotoxic Potential of Sterculia quadrifida Leaf Extract Against Human Breast Cancer Cell Lines

Rollando Rollando*, Eva Monica, Muhammad H. Aftoni
Department of Pharmacy, Faculty of Science and Technology, Ma Chung University, Malang, Indonesia

Corresponding Author: [email protected]; Tel: +6282220379864
Recieved Date: 15 July 2022; Accepted Date: 31 August 2022; Published Date: 03 September
Citation: Rollando R, Monica E, Aftoni MH. In vitro Cytotoxic Potential of Sterculia quadrifida Leaf Extract Against Human Breast Cancer Cell Lines. Trop J Nat Prod Res. 2022; 6(8):1228-1232. http://www.doi.org/10.26538/tjnpr/v6i8.12
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© 2022 Rollando et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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ABSTRACT

Breast cancer is the most common type of cancer among women worldwide. The main treatments for breast cancer are surgery and chemotherapy, but the medications used are associated with several side effects. Sterculia quadrifida R. Br is traditionally used by the people of East Nusa Tenggara as a medicinal plant to treat various diseases. This study was conducted to determine the potential of the ethanol fraction of S. quadrifida leaf extract as an anti-breast cancer agent. Human breast cancer cell lines (MCF-7, T47D, 4T1, MDA-MB-231, and Vero) were obtained for the study. An ethanol solvent (80%) was employed to extract the leaves of S. quadrifida using the maceration method. The S. quadrifida leaf extract was then fractionated with n-hexane, ethyl acetate, and n-butanol with a liquid-liquid partition. An in vitro cytotoxicity assay was conducted on the MCF-7, T47D, 4T1, MDA-MB-231, and Vero cells using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) method. The results showed that the ethyl acetate fraction of the S. quadrifida leaf extract exhibited considerable cytotoxic activity in T47D and 4T1 breast cancer cells, with an IC50 value of 19.86 ± 3.48 and 16.70 ± 3.33 ?g/mL, respectively. Furthermore, the n-hexane fraction had the highest activity in MCF-7 cells with an IC50 value of 33.78 ± 7.82 ?g/mL. The findings of this study revealed that the leaves of S. quadrifida have the potential to act as a cytotoxic agent on breast cancer cells.

Keywords: Breast cancer, Cytotoxic, Leaf extract, MTT assay, Sterculia quadrifida
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
Index Copernicus Value (ICV) for 2017: 59.83
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