Euphorbia milii: Phenolic contents, Cytotoxic, and Antioxidant Prospect

Khuram Ashfaq1*, Muhammad A. Rehman2, M. Abuzar Ghaffari3, Nasir Ali3, Nayab Sohail3
1Department of pharmaceutical chemistry, Lahore Pharmacy College, Lahore, Pakistan
2Ruth Pfau College of Nutrition Sciences, LMDC, Lahore, Pakistan
3Department of pharmaceutical chemistry, Lahore Pharmacy College, Pakistan

Corresponding Author: [email protected]; Tel: 00923336892628
Recieved Date: 31 May 2022; Accepted Date: 29 August 2022; Published Date: 03 September
Citation: Ashfaq K, Rehman MAU, Ghaffari AM, Ali N, Sohail N. Euphorbia milii: Phenolic contents, Cytotoxic, and Antioxidant Prospect. Trop J Nat Prod Res. 2022; 6(8):1219-1222.
© 2022 Ashfaq et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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The development of pharmacopeial, non-pharmacopeial, or synthetic medications has extensive relied on exploiting medicinal plants as they are a rich source of medicinal components. The present study focused on examining the cytotoxic, phenolic, and antioxidant prospects of Euphorbia milii. The whole dried plant was extracted using n-hexane, dichloromethane, and methanol to produce the organic extracts. Total phenolic contents, cytotoxic contents, and antioxidant activities were measured using the Folin–Ciocalteu reagent method, brine shrimp lethality bioassay, and DPPH radical scavenging assay, respectively. Anthraquinones, saponins, cardiac glycosides, and flavonoids were identified by phytochemical analysis. The highest phenolic content (276.41 ± 64 mg GAE/g dry extract wt) was observed in the methanol extract. Significant (P<0.04) antioxidant potential was exhibited by the methanol extract (78.8% inhibition with IC50 value of 35.71µg/mL). Methanol extract showed notable cytotoxicity at the highest dose level in the brine shrimp lethality test, with an LD50 of 471.05 (µg/mL). The study found considerable antioxidant and cytotoxic properties in Euphorbia milii. Further study is advised to identify the secondary metabolites responsible for the biological activities described.

Keywords: Euporbia milii, Total phenolics, Antioxidant, Cytotoxic.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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