Development and Validation of HPLC Method for Determination of Quercetin in Hydrogel Transdermal Patches Loaded with Red Onion Peel Extract

Wattanaporn Phattanaphakdee1, Chadartharn Ditipaeng1, Pimpon Uttayarat2, Thanu Thongnopkoon3, Sirivan Athikomkulchai4*,  Chuda Chittasupho5*
1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Srinakharinwirot University, Nakhon Nayok 26120, Thailand
2Nuclear Technology Research and Development Center, Thailand Institute of Nuclear Technology (Public Organization), Nakhon Nayok 26120, Thailand
3Department of Pharmaceutical Technology, Faculty of Pharmacy, Srinakharinwirot University, Nakhon Nayok 26120, Thailand
4Department of Pharmacognosy, Faculty of Pharmacy, Srinakharinwirot University, Nakhon Nayok 26120, Thailand
5Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand

Corresponding Author: [email protected], [email protected]; Tel: +66897796744, +6653944390
Recieved Date: 23 May 2022; Accepted Date: 28 August 2022; Published Date: 03 September
Citation: Phattanaphakdee W, Ditipaeng C, Uttayarat P, Thongnopkoon T, Athikomkulchai S, Chittasupho C. Development and Validation of HPLC Method for Determination of Quercetin in Hydrogel Transdermal Patches Loaded with Red Onion Peel Extract. Trop J Nat Prod Res. 2022; 6(8):1210-1214. http://www.doi.org/10.26538/tjnpr/v6i8.8
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© 2022 Phattanaphakdee et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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ABSTRACT

Quercetin is the major flavonoid present in red onion (Allium cepa L.). The determination of quercetin in red onion extract has been investigated previously. However, the HPLC method for analyzing quercetin in hydrogel transdermal patches with red onion peel extract has not been reported. This study aimed to develop and validate the novel HPLC methods for the determination of quercetin in hydrogel transdermal patches loaded with red onion peel extract. The HPLC condition and system configuration were optimized to obtain acceptable validation parameters. Method validations were performed for the quantitative analyses of quercetin in hydrogel transdermal patches with red onion peel extract. The reversed phase HPLC analytical method was developed. The C18 column and 100% acetonitrile and 0.1% aqueous phosphoric acid solution as a mobile phase were applied at a flow rate of 1.0 mL/min with gradient elution. The injection volume was 10 µL. The separation was performed at ambient temperature with UV detection at 364 nm. The retention time of quercetin was 23.9 min, with a total run time of 27 min. The linearity range of calibration curves was 5-150 µg/mL (R2>0.999). The limit of detection (LOD) and limit of quantification (LOQ) were 0.18 and 0.53 µg/mL, respectively. The mean recoveries were reported as 99.35–108.29%, indicating accuracy of the method. The %RSD study was less than 2.0%, suggesting the precision of the method. This method successfully determined quercetin in hydrogel transdermal patches with red onion peel extract.

Keywords: Quercetin, HPLC, Hydrogel, Transdermal, Red onion peel.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
Index Copernicus Value (ICV) for 2017: 59.83
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