Effect of Propolis Extract on Oxidative Stress Biomarker in Diabetic Wistar Rat (Rattus novergicus)

Nur S. Meida1,2*, Bambang Purwanto1, Brian Wasita1, Senyum Indrakila1, Soetrisno Soetrisno1, Eti Poncorini1, Risya Cilmiaty1, Woro R. Pratiwi3, Yunani Setyandriana2, Syifa Almahira2
1Medical Science, Universitas Sebelas Maret Surakarta, Indonesia
2Medical Education Study Program, Faculty of Medicine and Health Sciences, Universitas Muhammadiyah Yogyakarta, Yogyakarta, Indonesia
3Department of Pharmacology and Therapy, Universitas Gadjah Mada, Yogyakarta, Indonesia

Corresponding Author: [email protected] ; Tel: +6281329877805
Recieved Date: 02 May 2022; Accepted Date: 28 August 2022; Published Date: 03 September
Citation: Meida NS, Purwanto B, Wasita B, Senyum Indrakila S, Soetrisno, S, Poncorini E, Cilmiaty R, Pratiwi WR, Setyandriana Y, Almahira S. Effect of Propolis Extract on Oxidative Stress Biomarker in Diabetic Wistar Rat (Rattus novergicus). Trop J Nat Prod Res. 2022; 6(8):1193-1196. http://www.doi.org/10.26538/tjnpr/v6i8.5
© 2022 Meida et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Diabetes mellitus (DM), is a hyperglycemic condition, which increases oxidative stress, thereby leading to complications. One of the biomarkers of oxidative stress is malondialdehyde (MDA), whose levels can be used to predict oxidative stress levels. Propolis, an herbal medicinal plant extensively used to treat a variety of ailments, has been shown to lower oxidative stress. This study was conducted to determine the effect of Gunung Lawu propolis extract on MDA levels in a diabetic rat model. Ethanol extract of Gunung Lawu propolis was prepared and used in the treatment. Twenty-five (25) Wistar rats (Rattus novergicus) were randomly chosen and divided into five groups. The normal (healthy control; K1), DM (positive control; K2), and treatment groups, which consisted of DM rats that received different doses of propolis extract: P1 (100 mg/kg BW after 14 days of induction), P2 (200 mg/kg BW after 14 days of induction), and P3 (200 mg/kg BW immediately after induction). After 28 days, the rats were euthanized, and blood samples were collected and processed into the serum. The levels of MDA in the blood serum samples were measured. The results revealed that propolis significantly reduced fasting blood sugar (p<0.01) and MDA levels (p<0.1) in diabetic rats. Propolis administration did not show a significant difference (p>0.01) in reducing fasting blood sugar and MDA levels in early (P3) and advanced (P2) diabetes stages. The findings of this study revealed that propolis can lower MDA levels (an oxidative stress biomarker) in diabetic rats, thereby suggesting its potential in managing DM.

Keywords: Diabetes mellitus, MDA, Oxidative stress, Propolis.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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