In vitro Inhibitory HMG-CoA Reductase Activity of Purified Polyphenol Compounds from Water Lettuce (Pistia Stratiotes) Leaf Extract

Sabri Sudirman*, Miftahul Janna, Herpandi, Indah Widiastuti
Fisheries Product Technology, Faculty of Agriculture, Universitas Sriwijaya, Indralaya 30862, Ogan Ilir Regency, South Sumatra, Indonesia
Corresponding Author: [email protected]; Tel: +62 711580934
Recieved Date: 02 June 2022; Accepted Date: 23 July 2022; Published Date: 04 August 2022
Citation: Sudirman S, Janna M, Herpandi, Indah Widiastuti I. In vitro Inhibitory HMG-CoA Reductase Activity of Purified Polyphenol Compounds from Water Lettuce (Pistia Stratiotes) Leaf Extract. Trop J Nat Prod Res. 2022; 6(7): 1131-1134 http://www.doi.org/10.26538/tjnpr/v6i7.15
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© 2022 Sudirman et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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ABSTRACT

The 3-hydroxy-3-methylglutaryl-coenzyme-A (HMG-CoA) reductase is an enzyme that plays a role in the synthesis of cholesterol. The side effect of synthetic anti-cholesterol drugs has led to the demand for natural HMG-CoA reductase inhibitors such as plant extract. Therefore, this study aimed to determine the inhibitory HMG-CoA reductase activity of polyphenol compounds from water lettuce (Pistia stratiotes) leaf extract. The research was conducted using the crude and purified extracts of water lettuce. The total polyphenols, flavonoids, and HMG-CoA reductase inhibitory activity assay were carried out by in vitro analysis. The values obtained were analyzed quantitatively, followed by the use of an independent sample t-test and presented in graphical form. The results showed that the total polyphenols in each crude and purified extracts were 29.03 mg GAE/g dry sample and 65.63 mg GAE/g dry sample, respectively, while the total flavonoids were 27.58 mg QE/g dry sample and 88.02 mg QE/g dry sample, respectively. The inhibitory activity of the HMG-CoA reductase enzyme showed that the purified extract showed percentage inhibition of 34.74% which was higher than that of the crude extract which was 2.61%. This indicated that the purified extract of water lettuce has higher levels of polyphenols and flavonoids that can inhibit the HMG-CoA reductase enzyme more effectively than the crude extract.

Keywords: Anti-hypercholesterolemia, HMG-CoA reductase, Polyphenol, Water lettuce.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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