Isolation and Evaluation of Pharmacological Activities of a Bioactive Hydroxylated C28 Steroid from the Leaf of Laportea decumana (Roxb.) Wedd

Rollando Rollando*, Eva Monica, Muhammad H. Aftoni, Cristopher D. Kurniawan, Rehmadanta Sitepu

Department of Pharmacy, Faculty of Science and Technology, Ma Chung University, Malang 65151, Indonesia

Corresponding Author: [email protected]; Tel: +6282220379864

Recieved Date: 22 April; Accepted Date: 25 July ; Published Date: 04 August 2022

Citation: Rollando R, Monica E, Aftoni MH, Kurniawan CD, Sitepu R. Isolation and Evaluation of Pharmacological Activities of a Bioactive Hydroxylated C28 Steroid from the Leaf of Laportea decumana (Roxb.) Wedd. Trop J Nat Prod Res. 2022; 6(7): 1096-1102. http://www.doi.org/10.26538/tjnpr/v6i7.9

Copyright:

© 2022 Rollando et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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ABSTRACT

Cancer and infection rates have risen significantly in the last decade. Medicinal plants can be used to treat cancer and infections. Laportea decumana (Roxb.) Wedd for example, has long been used as a medicinal plant, but scientific evidence on its pharmacological effects is limited. The present study was therefore aimed at isolating a bioactive compound from the leaves of Laportea decumana and evaluating its pharmacological activities. Ethanol was used to extract the leaves of L. decumana using the maceration method. Preparative thin layer chromatography (TLC) was used for the isolation of an active compound, and preparative high-performance liquid chromatography (HPLC) was employed for purification. The structure of the active compound was determined by UV/Vis, IR, MS, 1H-NMR, 13C-NMR, HMQC, and HMBC. Staphylococcus aureus and Escherichia coli were used to examine the antibacterial activity of the compound. Furthermore, the antioxidant potential of the new compound was determined by the DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. Molecular docking was performed with the PyRx program. Also, the compound was tested for its ability to inhibit 4T1 cancer cells. The results showed that the extraction process yielded an oily green, thick extract with a yield value of 5.19%. From the leaves of L. decumana, a new hydroxylated C28 steroid was isolated (MCI). The bioactive compound possessed antibacterial properties against the test organisms, as well as antioxidant activity by DPPH assay. Furthermore, MCI was active against 4T1 cancer cells. The findings of this study suggest that MCI has the potential as a cancer cell inhibitor.