In vitro Effects of Persea americana Aqueous Extracts Against Oxidants and Fe2+-Induced Oxidative Stress in Rats’ Pancreas

Temitope I. Adelusi1*, Ganiyu Oboh2, Ayodele J. Akinyemi3
1Department of Biochemistry, Ladoke Akintola University of Technology, Nigeria.
2Department of Biochemistry, Federal University of Technology, Akure, Nigeria.
3Department of Biochemistry, Afe Babalola University, Ado Ekiti, Nigeria.

Corresponding Author: [email protected]; Tel: +8615050827942
Recieved Date: May 19, 2018; Accepted Date: June 07, 2018; Published Date: 07 June 2018
Citation: Adelusi TI, Oboh G, Akinyemi AJ. In vitro Effects of Persea americana Aqueous Extracts Against Oxidants and Fe2+-Induced Oxidative Stress in Rats’ Pancreas. Trop J Nat Prod Res. 2018; 2(6):293-302.
Copyright: © 2018 Adelusi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Various mechanisms have been reported to orchestrate the antioxidant properties of bioactive compositions of medicinal plants in the management/treatment of many diseases. In this research, we focused on the antioxidant and anti-lipid peroxidation potential of aqueous extracts of Persea americana. Total phenol content, total flavonoid content, ferric reducing antioxidant property (FRAP), OH- radical scavenging ability, 1, 1-diphenylpicryhydrazyl (DPPH) radical scavenging ability and anti-lipid peroxidation strength of the extracts were estimated using 5 mg/mL. Leaf and seed extracts showed the highest total phenol and flavonoid contents respectively, while the FRAP of the seed was significantly (P < 0.05) higher than others. IC50 values revealed that Peel (IC50 = 0.335 ± 0.067) has the highest DPPH potential while OH- radicals and MDA were significantly (P < 0.05) reduced by the leaf extract in a dose dependent pattern. Characterization of avocado leaf and fruit parts revealed varied concentrations of phenolics (Syringic acid, eugenol, vnillic acid, isoeugenol, guaiacol, phenol, kaempherol, catechin, ?-hydroxybenzoic acid, ferulic acid, apigenin, naringenin, epigallocatechin, lupeol and epigallocatechin-3-O-gallate). Our findings showed that the aqueous extract of the leaf and fruit parts of avocado pear (P. americana) protected the pancreas from Fe2+ induced lipid peroxidation in vitro which could be attributed to their phenolic compounds and this may be due to their antioxidant activities, Fe2+ chelating ability, radical scavenging strength and reducing power. This research therefore suggests the use of avocado pear as a functional food for the management of oxidative stress related diseases.

Keywords: Persea americana, lipid peroxidation, malondialdehyde, phenolic compounds, antioxidant activities.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
Index Copernicus Value (ICV) for 2017: 59.83
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