Effects of Costus afer Extract in Mouse Models of Anxiety and Depression and Its Possible Mechanisms of Action

Akanji A. Murtala1,2, Abidemi J. Akindele1*, Ibrahim A. Oreagba1
1Department of Pharmacology, Therapeutics & Toxicology, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Lagos, Nigeria
2Department of Pharmacology, Faculty of Basic Medical Sciences, Obafemi Awolowo College of Health Sciences, Olabisi Onabanjo University, Sagamu, Ogun State, Nigeria

Corresponding Author: [email protected].edu.ng, [email protected]; Tel: +2348036357197
Recieved Date: 5 March 2022; Accepted Date: 13 April 2022; Published Date: 03 May
Citation: Murtala AA, Akindele AJ, Oreagba IA. Effects of Costus afer Extract in Mouse Models of Anxiety and Depression and Its Possible Mechanisms of Action. Trop J Nat Prod Res, 2022; 6(4): 654-660. http://www.doi.org/10.26538/tjnpr/v6i4.30
© 2022 Murtala et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Costus afer (Costaceae) is a perennial rhizomatous plant found in tropical Africa. It is used in traditional medicine to treat central nervous system disorders. The study investigated the anxiolytic- and antidepressant-like effects of the hydroethanol leaf extract of Costus afer and its possible mechanism(s) of action in mice. C. afer (25-200 mg/kg, p.o.), distilled water (10 mL/kg, p.o.), diazepam (1 and 3 mg/kg, p.o.) and imipramine (20 mg/kg, p.o.) were given 1 h before various tests, including hole-board, open field, elevated plus maze, light/dark exploration (anxiolytic-like activity), forced swim (FST) and tail suspension (TST) (antidepressant-like effect) tests. C. afer (50-200 mg/kg) increased number of head dips (hole-board test; p < 0.05), entries and dips in open arms (elevated plus maze test; p < 0.05-0.001), general square crossings (open field test; p < 0.05) and decreased time spent in the dark box (light/dark exploration test; p < 0.05). C. afer, with peak effect observed at 200 mg/kg, increased (p < 0.01) the latency of immobility and decreased (p < 0.001) the duration of immobility in both FST and TST. Sulpiride (dopamine D2 receptor antagonist, 50 mg/kg), prazosin (?1-adrenoceptor antagonist, 1 µg/kg) and metergoline (5-HT2 receptor antagonist, 4 mg/kg) significantly (p < 0.05-0.01) blocked the anti-immobility effect of C. afer in FST. Findings showed that C. afer possess anxiolytic- and antidepressant-like activities, possibly mediated by ?1-adrenergic, dopamine D2 and 5-HT2 receptors.

Keywords: Costus afer, Costaceae, Anxiolytic, Antidepressant, Mental disorders, Imipramine.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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