Combination of High-Performance Liquid Chromatography (HPLC) Methods for Isolation of Ampelopsin C, a Trimeric Oligostilbene

Nur Atikah M. Asri¹, Mazlyana M. Zainul², Nurhuda Manshoor^1,2*

¹Faculty of Pharmacy, Universiti Teknologi MARA Selangor, Kampus Puncak Alam, 42300 Selangor, Malaysia

 ²Atta-ur-Rahman Institute for Natural Products Discovery, Universiti Teknologi MARA Selangor, Kampus Puncak Alam, 42300 Selangor, Malaysia

Corresponding Author: [email protected]; Tel: +603-32584715

Recieved Date: 02 February 2022; Accepted Date: 14 April 2022; Published Date: 03 May

Citation: Asri NAM, Zainul MM, Manshoor N. Combination of High-Performance Liquid Chromatography (HPLC) Methods for Isolation of Ampelopsin C, a Trimeric Oligostilbene. Trop J Nat Prod Res. 2022; 6(4): 537-541 http://www.doi.org/10.26538/tjnpr/v6i4.12

Copyright:

© 2022 Asri et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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ABSTRACT

Ampelopsin C is a trimeric oligostilbene found in a limited number of plant families including Dipterocarpaceae. It shows anti-inflammatory effects in experimental models of psoriasis, induces apoptosis in HepG2 human hepatoma cell line, inhibits human glioma through inducing apoptosis and autophagy and other biological properties. The present work oversees a method for isolation of ampelopsin C from the methanol extract of Dipterocarpus semivestitus leaves. Different chromatographic techniques were employed, including an ultra-high performance liquid chromatography (UHPLC) analysis for method development, a preparative high performance liquid chromatography (prep-HPLC) for isolation and a recycling high performance liquid chromatography (r-HPLC) for purification of the compound. The isolated compound was identified and characterized using nuclear magnetic resonance (NMR). The spectroscopic data confirmed the structure of ampelopsin C.