Total Phenolic, Total Flavonoid Contents, and Radical Acavenging Activity of Different Concentrations of Ethanol Extract of Pyrrosia piloselloides (L) M. G. Price

Djarot S.H. Seno¹, Dewi P.D.B. Sembiring¹, Popi A. Kurniatin¹, Waras Nurcholis^1,2*

¹Department of Biochemistry, Faculty of Mathematic and Natural Science, Bogor Agricultural University, Bogor, Indonesia

²Tropical Biopharmaca Research Center, Bogor Agricultural University, Bogor, Indonesia

Corresponding Author: [email protected] ; Tel: +62-8179825145

Recieved Date: 18 March 2022; Accepted Date: 25 April 2022; Published Date: 03 May

Citation: Seno DSH, Sembiring DPDB, Kurniatin PA, Nurcholis W. Total Phenolic, Total Flavonoid Contents, and Radical Acavenging Activity of Different Concentrations of Ethanol Extract of Pyrrosia piloselloides (L) M. G. Price. Trop J Nat Prod Res. 2022; 6(4): 484-487 http://www.doi.org/10.26538/tjnpr/v6i4.5

Copyright:

© 2022 Seno et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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ABSTRACT

Pyrrosia piloselloides (L.) M.G. Price (PP) is known to have pharmacological activities. This study investigated the total phenolic (TPC), total flavonoid contents (TFC), and the radical scavenging activities (RSA) of different concentrations of PP ethanol extract. PP dried sample was macerated with different ethanol concentrations (30%, 50%, and 70%). The TPC and TFC were determined using Folin-Ciocalteu and AlCl₃ calorimetric assays, respectively while the RSA was investigated using the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method. It was discovered that TPC (9.74 mg GAE/g), TFC (6.21 mg QE/g), and RSA (73.50%) of 50% ethanol extract of PP were higher than extracts obtained with 30% and 70% ethanol. Furthermore, a positive correlation was found between RSA vs TPC (r=0.43) and RSA vs TFC (r=0.35). It was concluded that 50% ethanol could be the best ethanol concentration to obtain PP extract with high contents of TPC, TFC, and antioxidant properties.