Green Synthesis of Silver Nanoparticles Using Cola nitida Nut Extract (Vent.) Schott & Endl. (Malvaceae), Characterization and the Determination of their Antimicrobial Activity

Nnenna B. Omeche1, Ifeyinwa C. Ezeala2, ⃰, Chinedu J. Ikem3, Philip F. Uzor1,2 ⃰
1Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Madonna University Elele, Rivers State, Nigeria
2Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, University of Nigeria, Nsukka 410001 Enugu, Nigeria
3Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Madonna University, Elele, Rivers State, Nigeria










Corresponding Author: [email protected]; [email protected]; Tel: +2348063459642
Recieved Date: 03 December 2021; Accepted Date: 28 January 2022; Published Date: 03 January
Citation: Omeche NB, Ezeala IC, Ikem CJ, Uzor PF. Green Synthesis of Silver Nanoparticles Using Cola nitida Nut Extract (Vent.) Schott & Endl. (Malvaceae), Characterization and the Determination of their Antimicrobial Activity. Trop J Nat Prod Res. 2022; 6(1):156-160 http://www.doi.org/10.26538/tjnpr/v6i1.25
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© 2022 Omeche et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.














ABSTRACT

Pathogenic microorganisms have developed resistance to existing antibiotics at an alarming rate; this has encouraged researchers to make a shift to the development of new drugs. One of the new developments used is the synthesis of silver nanoparticles (AgNPs). The aim of this study was to characterize and evaluate the antimicrobial activity of silver nanoparticle synthesized using Cola nitida nut extract. The AgNPs were synthesized by mixing 150 mL of silver nitrate solution (1 mM) with 750 mL of aqueous extract of Cola nitida nut at room temperature. Characterization of the synthesized AgNPs was done by UV-VIS, FTIR and particle size analysis. AgNPs were screened against Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella typhi, Candida albicans and Aspergillus niger. The result of the UV-VIS analysis showed the maximum absorbance wavelength of 439 nm at 4.5 h while FTIR showed the presence of functional groups such as alcohols and amides in the AgNPs. The particle size analysis showed that the average particle size was 227.2 nm. The antimicrobial screening showed that the AgNPs have activity against P. aeruginosa and B. subtilis with inhibition zone diameter of 8 mm and 6 mm respectively. The minimum inhibitory concentration (MIC) against S. typhi, S. aureus and C. albicans was 9 µg/mL for each of the organisms. The MIC for each of E. coli and A. niger was 10 µg/mL while that of B. subtilis was 7 µg/mL. The synthesized silver nanoparticles were found to be stable and effective against the microorganisms.

Keywords: Silver nanoparticles, Green synthesis, Cola nitida, Ultraviolet-Visible spectrophotometer, FTIR.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
Index Copernicus Value (ICV) for 2017: 59.83
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