Screening of Five Herbal Formulations Sold in South-East Nigeria for their Phytochemical Properties, In Vitro Antioxidant, Antiplasmodial and Cytotoxic Activities

Chinedu J. Ikem1,2*, Angus N. Oli2, Ebenezer Ofori-Attah3, Abigail Aning3, Regina Appiah-Opong3, Charles O. Esimone2
1Department of Pharmaceutical Microbiology and  Biotechnology, Faculty of Pharmacy, Madonna University, Elele, River State, Nigeria 
2Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Nigeria 
3Department of Clinical Pathology, Noguchi Memorial Institute for Medical Research, University of Ghana









Corresponding Author: [email protected]; [email protected]; Tel: +2348062996055
Recieved Date: 21 December 2021; Accepted Date: 01 February 2022; Published Date: 03 January
Citation: Ikem CJ, Oli AN, Ofori-Attah E, Aning A, Appiah-Opong R, Esimone CO. Screening of five Herbal Formulations sold in South-east Nigeria for their Phytochemical Properties, In Vitro Antioxidant, Antiplasmodial and Cytotoxic Activities. Trop J Nat Prod Res. 2022; 6(1):150-155 http://www.doi.org/10.26538/tjnpr/v6i1.24
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© 2022 Ikem et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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ABSTRACT

Due to their rich bioactive secondary metabolites, herbal formulations sold in Nigeria are believed to have a curative effect on ailments including malaria. The study assessed the phytochemical properties, antioxidant properties, antiplasmodial, and cytotoxic effects of five commercially available herbal formulations. Preliminary phytochemical analysis for alkaloids, flavonoids, saponins, steroids, tannins, and terpenoids was carried out following standard procedures. The in vitro antiplasmodial assay was evaluated using the DD2 chloroquine resistant Plasmodium falciparum strain, while cytotoxicity was assessed using the tetrazolium-based colorimetric (MTT) assay, and the antioxidant properties were evaluated by assessing (2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) radical scavenging activities of these herbal formulations. Results revealed that the five herbal formulations had antiplasmodial potential and also had a cytotoxic effect on jukart cell lines used for the assay, and finally, there was variation in phytochemical constituents, total phenolic content, and antioxidant activity among the five herbal formulations screened. It can be concluded that these herbal formulations possess antimalarial potential and are not toxic to the Red blood cells.

Keywords: Herbal formulations, Phenolic, Antioxidant, Terpenoids, Antiplasmodial, Plasmodium.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
Index Copernicus Value (ICV) for 2017: 59.83
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