Antioxidant and Anti-inflammatory Activities of Extract and Fractions of Spondias mombin Leaf and Isolation of its Active Principles


Eze E. Ajaegbu1,2*, Ikemefuna C. Uzochukwu1, Festus B.C. Okoye1
1Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, 420281 Awka, Nigeria
2Department of Applied Sciences, Faculty of Pure and Applied Sciences, Federal College of Dental Technology and Therapy, Trans-Ekulu, Enugu State, Nigeria





Corresponding Author: [email protected]; Tel: +2348069744845
Recieved Date: 23 August 2021; Accepted Date: 20 January 2022; Published Date: 03 January
Citation:
Ajaegbu EE, Uzochukwu IC, Okoye FBC. Antioxidant and Anti-inflammatory Activities of Extract and Fractions of Spondias mombin Leaf and Isolation of its Active Principles. Trop J Nat Prod Res. 2022; 6(1):80-86

http://www.doi.org/10.26538/tjnpr/v6i1.15
Copyright:
© 2022 Ajaegbu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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ABSTRACT

Spondias mombin L. (Anacardiaceae) leaves have shown remarkable efficacy in the ethnomedicinal management of inflammatory disorders. This study aims to examine the antioxidant and anti-inflammatory potentials of the plant material as well as identify its major phenolic components. The crude methanol extract (CME) and fractions [n-hexane (HF), dichloromethane (DF), ethyl acetate (EF), acetone (AF) and methanol (MF) fractions] of S. mombin were assessed for their antioxidant and anti-inflammatory effects using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging method and egg-albumin induced rat paw edema model respectively. The total phenolic contents of the extract and fractions were quantified spectrophotometrically and some of the compounds were identified with the aid of HPLC, LC-ESI-MS/MS, and 1H-NMR. CME, HF, DF, EF, AF, and MF exhibited dose-dependent DPPH scavenging property with IC50 values of 20, 350, 21, 78, 60, and 8 ?g/mL, respectively. The activity of the methanol fraction was comparable to that of the regular drug, vitamin C (IC50 = 9.4 ?g/mL). The crude extract and fractions, at the dose of 400 mg/kg showed poor anti-inflammatory activity in the rat model. EF exhibited the highest content of phenolic compounds with a percent gallic acid equivalent of 20.57%. The plant phenolic compounds identified were mainly gallocatechin, kaempferol, and quercetin glycosides and their methoxy products as well as gallic and ellagic acid derivatives. The outcomes show that the leaves of S. mombin contain antioxidant phenolic compounds which could explain the remarkable efficacy in the ethnomedicinal management of oxidative stress, but had poor anti-inflammatory activity.

Keywords: Spondias mombin, Anti-inflammatory, Medicinal plant, Phenolic compounds, Free radicals.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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