In vivo Anti-Inflammatory Activity of Coleus atropurpureus Leaves Extract and Fractions

 Ipang Djunarko1,4, Nanang Fakhrudin2*, Arief Nurrochmad3, Subagus Wahyuono2
1Postgraduate Program of Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
2Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
3Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia 
4Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Sanata Dharma University Yogyakarta Campus III, 55282, Indonesia




Corresponding Author: [email protected]; Tel: +62 274 543120
Recieved Date: 19 November 2021; Accepted Date: 16 January 2022; Published Date: 03 January
Citation: Djunarko I, Fakhrudin N, Nurrochmad A, Wahyuono S. In vivo Anti-Inflammatory Activity of Coleus atropurpureus Leaves Extract and Fractions. Trop J Nat Prod Res. 2022; 6(1):40-43 http://www.doi.org/10.26538/tjnpr/v6i1.8
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© 2022 Djunarko  et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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ABSTRACT

Inflammation is a normal body response to injury. However, inflammation also contributes to the progression of various diseases including cancer, atherosclerosis, asthma, obesity, and rheumatoid arthritis. Thus, the discovery of antiinflammatory agents remains chalenging. Medicinal plant is a potential source of drug discovery, including for antiinflammatory agents. One of the medicinal plants traditionally used for treating inflammatory diseases is Coleus atropurpureus. This study evaluated the in vivo anti-inflammatory effect of Coleus atropurpureus leaves extract and its fractions. Wistar rats were divided into 5 groups: negative control (solvent treatment), positive control (Diclofenac 9 mg/kg BW), ethanol extract (EE), n-hexane fraction (HF), and ethanol-water fraction (EWF). The extract and fractions were given at the same dose (45 mg/kg BW) 6 h prior to the induction of inflammation using carrageenan. Thin layer chromatography (TLC) analysis was done to identify the chemical components of the extract and fractions. The ethanol extract (EE), n-hexane fraction (HF), and ethanol-water fraction (EWF) reduced paw oedema thickness by 23.66, 19.01, and 20.80%, respectively, compared to the negative control. TLC analysis revealed the difference in the phytochemical content of the fractions. The HF mainly contained terpenoids, whereas the EFW contained flavonoids. This study demonstrated that EE, HF, and EWF of C. atropurpureus leaves have antiinflammatory activity in carrageenan-induced paw edema in rats.

Keywords: Coleus, Paw oedema, Inflammation, Thin layer chromatography 
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
Index Copernicus Value (ICV) for 2017: 59.83
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