Phytochemical Analysis and Anti-Inflammatory Activity of Solanum dasyphyllum Schumach. & Thonn. Leaves Methanol Extract and its Fractions

Oladapo E. Oyinloye1,2, Abayomi M. Ajayi1,* Olusegun G. Ademowo1,3
1Department of Pharmacology and Therapeutics, Faculty of Basic Medical Sciences, University of Ibadan, Oyo-State, Nigeria
2Department of Pharmacology and Toxicology, Faculty of Pharmacy, Olabisi Onabanjo University, Ago-Iwoye, Ogun-State, Nigeria
3Institute for Advanced Medical Research and Training, College of Medicine, University of Ibadan, Oyo-State, Nigeria


Corresponding Author: [email protected]; Tel: +2348103861380
Recieved Date: 05 October 2021; Accepted Date: 31 December 2021; Published Date: 04 January
Citation: Oyinloye OE, Ajayi AM, Ademowo OG. Phytochemical Analysis and Anti-Inflammatory Activity of Solanum dasyphyllum Schumach. & Thonn. Leaves Methanol Extract and its Fractions. Trop J Nat Prod Res. 2021; 5(12): 2165-2169 http://www.doi.org/10.26538/tjnpr/v5i12.21
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 © 2021 Oyinloye et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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ABSTRACT

Solanum dasyphyllum (SD) leaf is used in ethnomedicine for treatment of gastrointestinal diseases, gout, swelling, pain and fever-related illnesses. There is however paucity of phytochemical and pharmacological reports on the leaves. Hence, this study was designed to investigate the phytochemical profile and anti-inflammatory activity of SD crude extract and fractions. Powdered SD leaf was extracted with 80% methanol (MESd), and partitioned sequentially into n-hexane (SdHXF), dichloromethane (SdDMF), ethyl-acetate (SdEAF), and aqueous fractions (SdAQF), respectively. Phytochemical screening and thin layer chromatography (TLC) was carried out on extract and fractions. Anti-inflammatory activity of extract/fractions were investigated using membrane stabilizing assay, carrageenan-induced rat paw oedema and hyperalgesia models.
Phytochemical screening revealed appreciable amount of alkaloids, anthraquinones, saponins, tannins, phenols and flavonoids in MESd while TLC spots revealed 13 points for SdHXF, 7 in SdDMF and 10 in SdEAF, respectively. The MESd and SdEAF showed significant anti-inflammatory activities in membrane stabilizing and in carrageenan-induced paw oedema and hyperalgesia models. In conclusion, bioassay guided evaluation of anti-inflammatory activity of Solanum dasyphyllum leaf demonstrated that the ethylacetate fraction demonstrated the most pronounced anti-inflammatory activity. Further study is needed to characterize bioactive constituents and demonstrate its mechanisms of anti-inflammatory activity.

Keywords: Solanum dasyphyllum, Bioactivity, Ethylacetate fraction, Anti-inflammatory.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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