Saccharomyces cerevisiae Strain – Growth Kinetics, Extracellular Enzymes and Production of Fermentable Sugars from a Range of Lignocellulose Residues

Egong J. Egong, Anene N. Moneke, Onyetugo C. Amadi, Tochukwu N. Nwagu, Chukwudi I. Nnamchi, Idiunnu P. Awodirin, Reginald C. Agu, Bartholomew N. Okolo

Department of Microbiology, Faculty of Biological Sciences, University of Nigeria Nsukka, Nigeria
Corresponding Author: [email protected] ; Tel: +234813419069
Recieved Date: 01 September 2021; Accepted Date: 04 December 2021; Published Date: 04 January
Citation: Egong EJ, Moneke AN, Amadi OC, Nwagu TN, Nnamchi CI, Awodirin IP, Agu RC, Okolo BN. Saccharomyces cerevisiae Strain – Growth Kinetics, Extracellular Enzymes and Production of Fermentable Sugars from a Range of Lignocellulose Residues. Trop J Nat Prod Res. 2021; 5(12): 2154-2159
© 2021 Egong et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Lignocellulosic materials are abundant in Nigeria and can be exploited for the production of industrially important enzymes by microorganisms. In this study, capacity of isolated Saccharomyces cerevisiae in producing crude enzymes and hydrolysis of selected renewable substrates – corn husks (CH) and Opete bagasse (OB) for sugar release was investigated. Fourier Transform Infrared Spectrophotometer (FTIR) was used to study the changes in the structures of the lignocellulosic substrates (CH and OB). High-Performance Liquid Chromatography (HPLC) was employed to study the effect of pretreatment on sugar release from CH and OB substrates. S. cerevisiae gave the highest cellulase (18.61 ± 2.35 U/gds), xylanase (54.79 ± 3.58 U/gds) and ligninase (110.79 ± 6.19 U/gds) activities after 6-day fermentation (SSF) at 28 ± 2oC. Maximum glucose production (44.83 ± 1.24 mg/gds) and (49.73 ± 1.88 mg/gds) respectively from pretreatment of opete bagasse (ENZTOB) and corn husk (ENZTCH) were obtained. Maximum glucose of 35.90 ± 1.24 mg/gds and 37.87 ± 2.49 mg/gds were obtained from untreated opete bagasse (ENZOB), and untreated corn husk (ENZCH), respectively. Inoculum/cell suspension released lower glucose levels – 28.887 ± 2.03 mg/gds and 38.362 ± 1.36 mg/gds for opete bagasse (IOB) and for corn husk (ICH) respectively. The xylose sugars obtained from pre-treated opete bagasse (ENZTOB), and corn husk (ENZTCH) were 55.91 ± 3.02 mg/gds and 42.38 ± 2.44 mg/gds, respectively and are higher than 24.364 ± 1.75 mg/gds and 29.349 ± 1.74 mg/gds obtained for untreated IOB and ICH, respectively. 

Keywords: Multienzymes, Saccharomyces cerevisiae, Waste biomass, Growth kinetics, Fermentable sugars.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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