Formulation and Evaluation of Antimicrobial Properties of Psidium guajava Ethanol Leaf Extract Creams

Sinodukoo E. Okafo1*, Felix O. Enwa2, Olayemi Amusile1
1Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Delta State University, Abraka, Nigeria
2Department of Pharmaceutical Microbiology and Biotechnology, Faculty of Pharmacy, Delta State University, Abraka, Nigeria


Corresponding Author: [email protected]; [email protected]; Tel: +234 8063386118
Recieved Date: 24 July 2021; Accepted Date: 29 December 2021; Published Date: 04 January
Citation: Okafo SE, Felix O. Enwa FO, Amusile O. Formulation and Evaluation of Antimicrobial Properties of Psidium guajava Ethanol Leaf Extract Creams. Trop J Nat Prod Res. 2021; 5(12): 2144-2148 http://www.doi.org/10.26538/tjnpr/v5i12.17
Copyright:
© 2021 Okafo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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ABSTRACT

Psidium guajava is a medicinal plant that belongs to the family Mytaceace. This study was carried out to evaluate the antimicrobial property of creams formulated using the ethanol extract of Psidium guajava leaf.
The powdered dried Psidium guajava leaf was extracted using cold maceration. The extract was concentrated and evaluated based on antimicrobial activity using test organisms; Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli, Staphylococcus aureus, Apergillus niger and Candida albicans. Herbal creams were formulated using the extract and they were evaluated based on antimicrobial and physicochemical properties.
Percentage yield for the extract was 53.4%w/w. The pH of the creams ranged from 5.1 to 6.4 while the viscosity ranged from 40000 to 77500 mPas. The spreadability was from 2.5±0.14 cm to 4.75±0.35 cm. The creams had good homogeneity and they were stable after three months accelerated stability study. The extract inhibited Pseudomonas aeruginosa, Bacillus subtilis, Escherichia coli and Staphylococcus aureus at 3.125 mg/ml with inhibition zone diameter (IZD) of 6 to 19 mm and Candida albicans at 25 mg/ml with IZD of 6 to 9 mm. The extract had no activity against Aspergillus niger at the tested concentrations (3.125 to 100 mg/ml). The IZD for the creams against the test bacteria ranged from 3.0±0.00 to 16±1.41 mm while IZD against Candida albicans was between 4.5±0.71 and 7.0±1.41 mm. The creams had no antifungal activity against Aspergillus niger.
This study showed that the extract and the formulated creams exhibited good antimicrobial activity against the test organisms with the exception of Aspergillus niger.

Keywords: Psidium guajava, Creams, Ethanol extract, Antimicrobials. 
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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