Neuroprotective Activity and Antioxidant Effect of Salacca zalacca Peel Ethanol Extract on High Glucose Induced Zebrafish (Danio rerio) Embryo

Husnul Khotimah¹, Wahyu E. Prima², Anditri Weningtyas^2*, Dea Aninditha², Safira N.P. Alita, Umi Kalsum¹, Shahdevi K. Nandar³, Masruroh Rahayu³, Dian Handayani⁴ 

¹Department of Pharmacology, Faculty of Medicine, Brawijaya University, Malang, Indonesia

²Master Program of Biomedical Sciences, Faculty of Medicine, Brawijaya University, Malang, Indonesia 

³Department of Neurology, Faculty of Medicine, Brawijaya University, Malang, Indonesia

^4,3Department of Nutrition, Faculty of Medicine, Brawijaya University, Malang, Indonesia

Corresponding Author: [email protected]; Tel: +6282245321199

Recieved Date: 04 September 2021; Accepted Date: 04 December 2021; Published Date: 04 January

Citation: Khotimah H, Prima E.W, Weningtyas A, Aninditha D, Alita SNP, Kalsum U, Nandar S.K, Rahayu M, Handayani D. Neuroprotective Activity and Antioxidant Effect of Salacca zalacca Peel Ethanol Extract on High Glucose Induced Zebrafish (Danio rerio) Embryo. Trop J Nat Prod Res. 2021; 5(12): 2079-2084 http://www.doi.org/10.26538/tjnpr/v5i12.6

Copyright:

© 2021 Khotimah et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Visit for more related articles at  Tropical Journal of Natural Product Research

ABSTRACT

Hyperglycemia in gestational diabetes (GDM) could lead to oxidative stress that result in reduction of superoxide dismutase (SOD) level and sirtuin (SIRT)-1 expression. Reduction of SOD and SIRT-1 inhibit CAMP response element-binding protein (CREB), a regulator of brain derived neurotrophic factor (BDNF) transcription, and reduces BDNF expression. Decreased BDNF expression in newborns could lead to autism spectrum disorder (ASD) and retinopathy of prematurity (ROP) which can interfere with memory and vision function during growth and development. Salacca zalacca (SZ) peel contains antioxidant neuroprotectant substances and this study was aimed to prove the effect of Salacca zalacca peel extract on the development of embryos induced by high glucose. The study used zebrafish embryos induced by 3% of glucose as a model of gestational diabetes. Salacca zalacca peel extract was given at concentrations of 0.1, 0.2, and 0.4 mg/mL. The expression of SIRT-1, BDNF, and SOD of zebrafish embryos was evaluated by reverse transcriptase PCR (RT-PCR). The results showed a significant increase in the expression of SIRT-1, BDNF, and SOD at the concentration of 0.4 mg/mL. It was concluded that Salacca zalacca peel extract has a neuroprotectant and antioxidant effects.