Acute Toxicity Studies of a Combination of Ethanol Extracts of Andrographis paniculata, Centella asiatica, and Curcuma heyneana in Rats

Dadang I. Husori^1*, Marianne¹, Popi Patilaya², Audrey N. Febrika¹

¹Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Padang Bulan, Medan 20155, Indonesia

²Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Padang Bulan, Medan 20155, Indonesia

Corresponding Author: [email protected]; Tel: +62618223558

Recieved Date: 27 May 2021; Accepted Date: 01 November 2021; Published Date: 04 January

Citation: Husori DI, Marianne, Patilaya P, Febrika AN. Acute Toxicity Studies of a Combination of Ethanol Extracts of Andrographis paniculata, Centella asiatica, and Curcuma heyneana in Rats. Trop J Nat Prod Res, 2021; 5(12): 2074-2078 http://www.doi.org/10.26538/tjnpr/v5i12.5

Copyright:

© 2021 Husori et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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ABSTRACT

Andrographis paniculata, Centella asiatica, and Curcuma heyneana are beneficial in the treatment of various diseases. A combination of extracts of several medicinal plants increases the potential of the expected effect. The present study was aimed at determining the acute toxicity of a combination of ethanol extracts of A. paniculata, C. asiatica, and C. heyneana in rats. A combination of ethanol extracts of the three plants was prepared. Twenty female rats were divided into 4 groups: the control group, and treatment groups of a combination of extracts at doses of 500, 2,000, and 5,000 mg/kg. The acute toxicity study was carried out on the experimental rats using the Organization for Economic Co-operation and Development (OECD) Guidelines for Testing of Chemicals number 423. The animals were sacrificed on the 15th day, and biochemical analyses were performed on the collected blood samples. Macroscopic and microscopic examinations of the vital organs were determined. The results revealed that there were no toxic symptoms in the female rats. The combination of extracts does not affect the body weight and the relative organ weight of the rats. Also, the biochemical parameters indicated that alanine transaminase (ALT), aspartate transaminase (AST), and creatinine levels increased at the dose of 5,000 mg/kg BW with no significant difference in AST level. The lethal dose (LD50) of the combination of the extracts is higher than 5,000 mg/kg BW. This study found that combining extracts of A. paniculata, C. asiatica, and C. heyneana is relatively non-toxic, with an LD₅₀ greater than 5,000 mg/kg.