Antiobesity Activity of Extract, Fractions and Pure Compounds from Husk of Zea mays

Jude E. Okokon1*, Mandu E. Nyong1, Paul S. Thomas2, Akaninyene O. Daniel3, Godwin N. Enin4, John A. Udobang5
1Department of Pharmacology and Toxicology, Faculty of Pharmacy, University of Uyo, Uyo, Nigeria
2Department of Pharmacognosy and Natural Medicine, Faculty of Pharmacy, University of Uyo, Uyo, Nigeria
3Department of Pharmaceutical and Medicinal Chemistry, Faculty of Pharmacy, University of Uyo, Uyo, Nigeria
4Department of Chemistry, Faculty of Science, University of Uyo, Uyo, Nigeria
5Department of Clinical Pharmacology and Therapeutics, Faculty of Basic Clinical Sciences, University of Uyo, Uyo, Nigeria

Corresponding Author: [email protected]; Tel: +234-8023453678
Recieved Date: 08 September 2021; Accepted Date: 20 October 2021; Published Date: 03 November
Citation: Okokon JE, Nyong ME, Thomas PS, Daniel AO, Enin GN, Udobang JA. Antiobesity Activity of Extract, Fractions and Pure Compounds from Husk of Zea  mays.Trop J Nat Prod Res. 2021; 5(10): 1868-1875. http://www.doi.org/10.26538/tjnpr/v5i10.27
Copyright:
© 2021 Okokonet al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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ABSTRACT

Zea mays L. (Poacae) husk is used in Ibibio traditional medicine for the treatment of various ailments including diabetes mellitus, dyslipidemia and malaria. The aim of this study was to evaluate the husk extract and fractions for obesity preventive activity in rats fed on high fat diet (HFD) to validate its folkloric claims. The ethanol husk extract of Zea mays (187-561 mg/kg) and fractions (hexane, dichloromethane (DCM), ethyl acetate(EA) and methanol, 200 mg/kg) were investigated for in vivo antiobesity activity in HFD-fed rats for 42 day and in vitro inhibitory activity against porcine pancreastic lipase. Orlistat and cetilistat were used as standard drugs. Body weights, body mass index, antherogenic index, lipid profile, blood glucose level, liver function indices and antioxidant biomarkers were parameters measured. Isolation and identification of compounds from active fraction was carried out by column chromatography and Nuclear Magnetic Resonance (NMR) spectroscopy, respectively. The isolated compounds were tested for porcine pancreatic lipase inhibitory activity. The husk extract and fractions caused considerable reduction of serum lipid levels, body and organ weights, antherogenic indices, Blood glucose level, liver function parameters and also increased antioxidative markers levels with the DCM fraction exerting the highest activity. Stigmasterol and stigmasterylpalmitate isolated from DCM fraction demonstrated inhibitory activity against porcine pancreatic lipase. These results suggest that the husk extract of Zea mayspossesses obesity preventive potentials which lay credence to its usage in folkloric medicine in the treatment of dyslipidemia and related diseases.

Keywords: Zea mays, Antiobesity, Stigmasterol, Stigmasterylpalmitate, Stigmasteryl stearate, High fat diet.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
Index Copernicus Value (ICV) for 2017: 59.83
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