Effects of Tang Shen 2 Hao Fang, a Traditional Chinese Medicine, on Diabetic Nephropathy in Sprague–Dawley Rats

Anh N. Dang¹, Wang Yao-Guang¹, Zhao Xi¹, Zhang Jing¹, Hoang N. Nguyen^2*

¹Tianjin University Traditional Chinese Medicine, Teaching Hospital 1, Nephropathy Department, Tianjin 300381, China

²Department of Pharmacology, Vietnam Military Medical University, 160 Phung Hung street, Ha Dong district, Ha Noi, Vietnam

Corresponding Author: [email protected]; Tel: +84-38-268-9686

Recieved Date: 09 August 2021; Accepted Date: 16 October 2021; Published Date: 03 November

Citation: Dang AN, Yao-Guang W, Xi Z, Jing Z, Nguyen HN. Effects of Tang Shen 2 Hao Fang, a Traditional Chinese Medicine, on Diabetic Nephropathy in Sprague–Dawley Rats. Trop J Nat Prod Res. 2021; 5(10): 1828-1834. http://www.doi.org/10.26538/tjnpr/v5i10.20

Copyright:

© 2021 Dang et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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ABSTRACT

Diabetic nephropathy is a chronic disease characterized by a reduced glomerular filtration rate. Kidney disease is a microvascular complication of diabetes mellitus, which can eventually lead to end-stage renal disease and threaten patient health. This study aimed to evaluate the effects of Tang Shen 2 Hao Fang (TS2HF), a traditional Chinese medicine formulation, on rats with diabetic nephropathy induced by streptozotocin injection and a high-fat diet. Rats were given TS2HF at doses of 11.57 and 23.14 g/kg/day. Rat weight, blood glucose levels, and blood lipid indices were measured, and renal function was assessed by blood urea, creatinine, and 24-hour urine albumin. Antioxidant effects were evaluated via serum superoxide dismutase (SOD) activity and glutathione (GSH). The anti-inflammatory effects of TS2HF were evaluated by measuring the proinflammatory cytokines interleukin 6 (IL-6) and tumor necrosis factor ? (TNF-????) using quantitative ELISA. The kidney weights and kidney/body weight ratios were also determined. The results demonstrated that TS2HF reduced kidney injury complications. TS2HF decreased triglycerides, total cholesterol, and low-density lipoprotein (LDL) and increased high-density lipoprotein (HDL). TS2HF decreased the cytokines IL-6 and TNF-???? and increased SOD and GSH levels in rat serum. It also protected against weight loss, decreased the glomerular size, decreased glomerular and interstitial fibrosis, and reduced the proliferation of endothelial and intercapillary cells in rats with diabetic nephropathy. These results suggest that TS2HF has anti diabetic and anti-nephropathic activities.