Assessment of Antioxidant, Antimicrobial, Cytotoxic Activities and Isolation of Some Chemical Constituents from Different Extracts of Pergularia daemia

Saleh B. Alghamdi1, Walid E. Abdallah2, Khaled A. Abdelshafeek2,3*

1Biology Department, Faculty of Science, Albaha University, Albaha, KSA  
2Chemistry of Medicinal Plants Department, Pharmaceutical Industries Division, National Research Centre, El Bohouth st. Dokki, Giza, P.O. 12622, Egypt
3Chemistry Department, Faculty of Science and Arts, AlMukhwah, Albaha University, KSA

Corresponding Author: [email protected]; Tel: 00201155470568
Recieved Date: 20 January 2021; Accepted Date: 21 October 2021; Published Date: 03 November
Citation: Alghamdi SB, Abdallah WE, Abdelshafeek KA. Assessment of Antioxidant, Antimicrobial, Cytotoxic Activities and Isolation of some Chemical Constituents from Different Extracts of Pergularia daemia. Trop J Nat Prod Res. 2021; 5(10): 1816-1827
© 2021 Alghamdi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Pergularia daemia is a wild plant growing in Albaha region. The aerial parts of P. daemia were extracted with water and organic solvents (chloroform, ethyl acetate, butanol, methanol, and petroleum ether). The chemical constituents of the unsaponifiable and the fatty acid fractions were identified using GC-MS analyses. The ethyl acetate and n-butanol extracts were subjected to chromatographic techniques. Total phenolic and flavonoid contents in the extracts were determined using colorimetric assay. The antioxidant activity was determined using 1,1-diphenyl-2-picrylhydrazyl free radical scavenging assay. The antimicrobial activity was evaluated using the modified Kirby-Bauer disc-diffusion method while the cytotoxic activity was evaluated using HepG-2 and HCT-116 cancer cell lines. Chromatographic separation of the ethyl acetate and n-butanol extracts afforded five flavonoidal compounds; apigenin, chyrsoeriol, quercetin, vitexin and lucenin-2.The total phenolic content of the extract ranged from 15.14 ± 0.02 to 39.33 ± 0.17 mg GAE/g. The ethyl acetate extract had the highest total flavonoid content (76.11 ± 0.16 mg QE/g) and the highest antioxidant activity (93.95%). The extracts showed inhibition against tested strains with the ethyl acetate extract having the maximum inhibition zone (17 to 23 mm against Staphylococcus aureus and Bacillus subtilis). The ethyl acetate extract showed a strong cytotoxicity activity against HEPG2 and HCT-116 cancer cell lines with IC50 values of 1.62 µg/mL and 2.48 µg/mL, respectively. This is the first report of the isolation of the five flavonoidal compounds from P. daemia. The plant has potential as antioxidant, antibacterial, and cytotoxic agents.

Keywords: Asclepiadaceae, Pergularia daemia, Antioxidant, Antimicrobial, Cytotoxic activity, Flavonoids
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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