Evaluation of Pharmacological Activities of Fractionated Extracts of Hoya parasitica Wall. Leaves

Kishore K. Sarkar*, Tamanna Islam, Trina Mitra, Asma Aktar, Iqbal M. Raja, Ibrahim Khalil, Md. Aktaruzzaman, Md. A. Rahman

Department of Pharmacy, Faculty of Biological Science and Technology, Jashore University of Science and Technology, Jashore-7408, Bangladesh

Corresponding Author: [email protected]; Tel: +8801737135110

Recieved Date: 08 June 2021; Accepted Date: 23 October 2021; Published Date: 03 November

Citation: Sarkar KK, Islam T, Mitra T, Aktar A, Raja IM, Khalil I, Aktaruzzaman M, Rahman MA. Evaluation of Pharmacological Activities of Fractionated Extracts of Hoya parasitica Wall. Leaves. Trop J Nat Prod Res. 2021; 5(10): 1747-1754. http://www.doi.org/10.26538/tjnpr/v5i10.9

Copyright:

© 2021 Sarkar et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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ABSTRACT

Hoya parasitica Wall. leaves are traditionally used to treat a number of ailments namely; rheumatism, kidney problems, jaundice, urinary tract disorder, constipation, fever, and pain. The purpose of the present study was to determine the phytochemical profile of n-hexane (NHPL) and ethyl acetate (EHPL) extracts of Hoya parasitica Wall leaves and to assess the neuropharmacological activities through elevated plus maze, open field, hole cross, and hole board tests together with cytotoxicity activity by brine shrimp lethality assay. In elevated plus maze test, significant time-dependent increase in anxiolytic effect was revealed by NHPL at a dose of 200 mg/kg whereas EHPL (200 mg/kg) showed significantly enhanced anxiolytic activity at 120 min compared to control (p<0.05). Moreover, in hole cross test, 200 mg/kg of NHPL demonstrated significant (p<0.05) dwindled locomotor activity during 5th observation period while time-dependent reduction of locomotor activity was exhibited by EHPL at a dose of 200 mg/kg. Again, in open field test, locomotor activity was found to be significantly dwindled by low dose of EHPL indicating sedative property whereas NHPL displayed significant sedative effect at the same dose at 30 min and 60 min compared to control (p<0.05). Head dipping activity was significantly (p<0.05) reduced in a dose-dependent manner by EHPL in hole board test which indicated CNS depressant effect. Furthermore, both extracts (200 mg/kg and 400 mg/kg) showed LC50 values of 172.874 µg/mL and 97.509 µg/mL, respectively. Therefore, the results of the present study disclose anxiolytic, sedative, CNS depressant and cytotoxic activities of Hoya parasitica Wall leaves.