Eco-Friendly and Cost-Effective Method for the Quantitative Determination of Ceftriaxone Sodium in Pharmaceutical Drug Formulations

Zeeshan Haider1, Zeeshan Ali1, Javaria Tayyab1, Muhammad M. Ahmad1, Muhammad Ahmad2, Muhammad Sajid1*, Dilshad Hussain3
1Institute of Chemical Sciences, Bahauddin Zakariya University, Multan, Pakistan
2Department of Chemistry, University of Sahiwal, Pakistan
3HEJ Research Institute of Chemistry, International Centre for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan


Corresponding Author: [email protected]; Tel: +923040801998
Recieved Date: 01 January 2021; Accepted Date: 18 October 2021; Published Date: 03 November
Citation: Haider Z, Ali Z, Tayyab A, Ahmad MM, Ahmad M, Sajid M, Hussain D. Eco-Friendly and Cost-Effective Method for the Quantitative Determination of Ceftriaxone Sodium in Pharmaceutical Drug Formulations. Trop J Nat Prod Res. 2021; 5(10): 1716-1723. http://www.doi.org/10.26538/tjnpr/v5i10.4
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© 2021 Haider et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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ABSTRACT

Ceftriaxone sodium is a beta-lactam antibiotic with bacteriocidal activity. This drug is eliminated in the urine, although only in a 33-67% concentration. Many High-Performance Liquid Chromatography (HPLC)-based methods for the analysis of ceftriaxone have been developed using various solvents, but these solvents are expensive, poisonous, and harmful. This study was therefore aimed at developing an eco-friendly and cost-effective HPLC-based approach for the quantitative determination of ceftriaxone sodium in pharmaceutical drug formulations. In the method development, the column used was X-Bridge C-18 (4.6 mm x 250 mm, 5 µm) for the separation, identification, and determination. The mobile phase was a mixture of phosphate buffer and methanol in the ratio of 73:27 (% v/v) at a flow rate of 1 mL/min, while the temperature was maintained at 25oC. Throughout the method development, the photodiode array detector was at a wavelength of 254 nm. The developed approach demonstrated good system applicability, with an RSD of less than 2%. R2 was 0.9998, which is exact because the % RSD is less than 2% (repeatability with 0.5% RSD and intermediate precision with 0.22% RSD). The limit of detection (LOD) and limit of quantification (LOQ) for the method were 0.0472 and 0.157 mg/mL, respectively. The R2 and RSD values suggested that the approach was linear, exact, and accurate, demonstrating that the developed method is environmentally friendly, cost-effective, and green.

Keywords: Ceftriaxone sodium, Cost-effective, Eco-friendly, High-Performance Liquid Chromatography, Method development, Pharmaceutical drug formulation.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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