In vitro Cytotoxic Activity of Ethyl Acetate Fraction of Hibiscus vitifolius Flowers Against HeLa Cell Line

Gujjula Nishitha, Gurram Asha Latha, Lagadapati Tejaswini, Amrutha Mounica, Kuruva Rekha, Nikitha K. Reddy, Bobillapati Jyothirmayi, Naga S. Kesana, Rahamat Unissa*

Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Malla Reddy College of Pharmacy, Maisammaguda, Dhulapally, Secunderabad, Telangana, India.

Corresponding Author: [email protected]; Tel: +91-7674085084

Recieved Date: December 18, 2017; Accepted Date: February 24, 2018; Published Date: 07 March 2018

Citation: Nishitha G, Latha AG, Tejaswini L, Mounica A, Rekha K, Reddy KN, Jyothirmayi B, Kesana SN, Unissa R. In vitro Cytotoxic Activity of Ethyl Acetate Fraction of Hibiscus vitifolius Flowers Against HeLa Cell Line. Trop J Nat Prod Res. 2018; 2(3):122-125.  https://doi.org//10.26538/tjnpr/v2i3.4

Copyright: © 2018 Nishitha et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

ABSTRACT

Hibiscus vitifolius Linn.(syn. Fioria vitifolia (L.) Mattei), is a plant native of India and many other tropical regions. It has been used traditionally for the treatment of a number of diseases. It is also well known for its antioxidant activity. The objective of the present study was to perform a preliminary phytochemical analysis and cytotoxic activity of ethyl acetate extract of Hibiscus vitifolius (Malavaceae) flowers. The finely powdered flowers were extracted successively with petroleum ether, diethyl ether and ethyl acetate in a soxhlet apparatus for 72 h. The ethyl acetate fraction obtained was tested for the phytochemical constituents by using preliminary phytochemical tests. The cytotoxic activity of the extract was screened by MTT (methylthiazolyl diphenyl- tetrazolium bromide) assay against HeLa (Human cervical cancer) cell lines. The phytochemical analysis revealed the presence of triterpenoids, steroids, flavonoids, tannins and phenolic compounds. The extract was shown to inhibit the cell growth with IC50 value of 81.27 µg/mL. These findings suggest that the ethyl acetate fraction obtained from the flower extracts of Hibiscus vitifolius has moderate cytotoxic activity.