Effects of Dipterocarpus alatus Leaf and Bark Extracts on UVB-Protection, Collagen Stimulating Activity and Nitric Oxide Inhibition

Oraya Lersprajak1, Nattawadee Kanpipit1, Natsajee Nualkaew2, Ploenthip Puthongking3, Suthasinee Thapphasaraphong3,4*

1Graduate School, Khon Kaen University, Khon Kaen 40002, Thailand
2Department of Pharmacognosy and Toxicology, Faculty of Pharmaceutical Science, Khon Kaen University, Khon Kaen, 40002, Thailand
3Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Khon Kaen University, 40002, Thailand
4Center for Research and Development of Herbal Health Products (CRD-HHP), Faculty of Pharmaceutical Sciences, Khon Kaen University 40002, Thailand

Corresponding Author: [email protected] ; Tel: +66-86-9218334
Recieved Date: 29 July 2021; Accepted Date: 22 September 2021; Published Date: 4 October
Citation: Lersprajak O, Kanpipit N, Nualkaew N, Puthongking P, Thapphasaraphong S. Effects of Dipterocarpus alatus Leaf and Bark Extracts on UVB-Protection, Collagen Stimulating Activity and Nitric Oxide Inhibition. Trop J Nat Prod Res, 2021; 5(9):1638-1644. http://www.doi.org/10.26538/tjnpr/v5i9.18
© 2021 Lersprajak et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Dipterocarpus alatus (D. alatus) is used for the treatment of rheumatism in Ayurvedic medicine. In this research, the leaf and bark ethanol extracts of D. alatus were investigated for antioxidant activity, UV-protection on HaCaT keratinocytes, inhibition of nitric oxide formation on RAW 264.7 macrophages and collagen proliferation on normal human dermal fibroblasts (NHDFs). The total phenolic and total flavonoid contents of the leaf and bark ethanol extracts were determined using Folin-Ciocalteu assay and AlCl3 assay, respectively. Cell viability of HaCaT keratinocytes, RAW 264.7 macrophages and normal human dermal fibroblasts (NHDFs) were evaluated including cell morphology, collagen production, nitric oxide inhibition. The leaf extract showed the highest phenolic content (1,327.07 ± 95.37 gGAE/100 g dry weight), flavonoid content (343.23 ± 21.36 gQE/100 g dry weight), and ferric reducing power (4.05 ± 0.34 mM FeSO4/100 g dried weight), whereas the bark extract showed better DPPH and ABTS assays (IC50 at 13.88 ± 0.27 and 28.15 ± 0.54 µg/mL, respectively). Both leaf and bark extracts showed no toxicity on HaCaT cells and NHDFs at 1-500 µg/mL, but the leaf extract above 25 µg/mL and bark extract above 100 µg/mL showed toxicity to Raw 264.7 cells. The leaf extract at 50 µg/mL presented more UVB protective effect than the bark extract with no toxicity to UVB-induced HaCaT cells. The bark extract induced higher collagen production and nitric oxide inhibition. The D. alatus showed beneficial potential for inclusion in UV protection products, whereas the bark extract is suited for wound healing products.

Keywords: Dipterocarpus alatus; Antioxidant activity UV protective effect; Wound healing effects Nitric oxide inhibition.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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