Phytochemical Analysis, Antioxidant and Anti-Acetylcholinesterase Activities of Jordanian Pistacia palaestina Bios Leaves Extract

Ahmed Al-Mustafa

1Biology Department, Faculty of Science, Mutah University, P.O. box 7, Karak, Jordan

Corresponding Author: [email protected]; Tel: 00962796711448
Recieved Date: 05 July 2021; Accepted Date: 13 September 2021; Published Date: 4 October
Citation: Al-Mustafa A. Phytochemical Analysis, Antioxidant and Anti-Acetylcholinesterase Activities of Jordanian Pistacia palaestina Bios Leaves Extract. Trop J Nat Prod Res. 2021; 5(9):1619-1625.
© 2021 Al-Mustafa. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Pistacia palaestina Boiss  is a medicinal plant with multiple dietary and therapeutic applications. The purpose of this work was to investigate the phytochemical content, the antioxidant, and the anti-acetylcholinesterase (AChE) activities of the P. palaestina leaves’ methanol extract. The antioxidant activity of the extract was tested by 2,2 -azino-bis-3 ethylbenzothiazoline-6-sulfonic acid (ABTS.+) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays, while the AChE inhibitory activity of the extract was determined according to Ellman’s assay. The composition of secondary phytochemical metabolites was established by HPLC-PDA and GC-MS analysis. P. palaestina leaves displayed strong ABTS•+ as well as DPPH radical scavenging ability with an IC50 value (6.86 µg/mL and 8.31 µg/mL) close to that of ascorbic acid (6.09 µg/mL and 6.96 µg/mL), respectively. For enzyme inhibition, the P. palaestina extract showed good activity against acetylcholinesterase in a dose-dependent manner with IC50 (57.67 ?g/mL) ten folds higher than the standard inhibitor galantamine (IC50 = 5.64 ?g/mL). The methanol extract displayed high phenolic (242.2 mgGAE/g extract) content. Similarly, the phytochemical profiling of the extract cautiously identified 19 different secondary metabolites, as accessed by HPLC and GC-MS analyses. Benzofenac methyl ester, 3,5-bis(1,1-dimethylethyl)- phenol, N-Dimethylaminomethyl-tertbutyl-isopropylphosphine, 14-methyl- Pentadecanoic acid methyl ester, 13-Tetradece-11-yn-1-ol, and Tridecane were the main compounds detected. Based on the current findings on the antioxidant and anti-AChE activities of P. palaestina methanol leaves extract, this plant may be recommended as a source of bioactive plant compounds and presents new alternatives for use in the management of neurological diseases.

Keywords: Pistacia palaestina, anticholinesterase, antioxidant, phenolic compounds, HPLC, GC-MS.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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