Phytochemical Investigation, Comparison and Characterization Study of Malaysian Stingless Bee Honey versus Jordanian Honey by LC-MS/MS

Nesrin Seder1, Walid A. Rayyan2*, Wael A. Dayyih3, Mohammad A. Al-Natour4, Abu Bakar Mohd Hilmi1*

1School of Biomedical Sciences, Faculty of Health Sciences, Universiti Sultan Zainal Abidin, 21300 Kuala Nerus, Terengganu, Malaysia
2Department of Medical Laboratory Analysis, Faculty of Science, Al-Balqa Applied University, Al-salt, Jordan.
3College of Pharmacy and Pharmaceutical Sciences/ Mutah University; Alkarak – Jordan.
4Pharmaceutical Medicinal Chemistry and Pharmacogonosy Department, Faculty of Pharmacy and Medical Sciences, University of Petra, Amman, Jordan.

Corresponding Author: [email protected]; [email protected] ; Tel: +962790272152
Recieved Date: 10 May 2021; Accepted Date: 18 September 2021; Published Date: 4 October
Citation: Seder N, Rayyan WA, Dayyih WA, Al-Natour MA, Mohd Hilmi AB. Phytochemical Investigation, Comparison and Characterization Study of Malaysian Stingless Bee Honey versus Jordanian Honey by LC-MS/MS. Trop J Nat Prod Res. 2021; 5(9):1597-1605.
© 2021 Seder et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Phytochemical studies have revealed more than 200 distinct polyphenolic compounds present in honey alone. Stingless bee honey (Trigona honey) is naturally found in tropical and subtropical regions. This study aims to interrogate the physical parameters, polyphenolic content, and antioxidant characteristics of Malaysian Trigona honey in comparison with Apis honey (Centaurea hyalolepis) and Citrus honey from Jordan. The three honey types were subjected to phytochemical and chromatographic analysis to explore the differences in honey composition related to bees type and geographical location. The total phenolic content ranged between 288.09 and 663.19 mg GAE/kg of honey in the three honey types. Trigona honey had the highest phenolic content (663.19 mgGAE/kg) followed by Centaurea hyalolepis honey (471.87 mgGAE/kg), both of which were higher than Citrus honey (288.09 mgGAE/kg). Trigona honey showed an IC50 of 61.042 ± 0.45 mg/mL, whereas, Centaurea hyalolepis honey and Citrus honey had IC50 of 120.29 ±  1.64 mg/mL and 129.51  ±  4.3 mg/mL, respectively. Statistical analysis has revealed a significant negative correlation between the IC50 value for the three honey samples and the concentration of polyphenols (p?0.001). Chromatographic analysis using LC-MS/MS showed a 28 and 42-fold difference in the polyphenolic content in Trigona over C. hyalolepis and Citrus honey, respectively. In conclusion, the diversity in the polyphenols contents and the high amounts of phytochemical compounds found in Trigona honey confers the antioxidant activity and there is no unique compound responsible for such activity over C. hyalolepis and Citrus honey.

Keywords: Trigona honey, Phytochemical, LC-MS/MS, Polyphenols, Jordan.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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