Botanical Features and Anti-cancerous Potential of Euphorbia nivulia Buch.-Ham

Muhammad Younus1,2*, Muhammad M. Hasan2, Muhammad Hanif 3, Shahzada K. Syed4 , Ghulam Sarwar5, Khalil Ahmad6, M. Sajid -ur- Rehman1, Jafir H. Shirazi7, Qazi A. Jamil7, Kashif ur Rehman Khan8, Abuzar Ghaffari 9, Muhammad S. Khan10, Muhammad Abdullah11, Muhammed A. Wazir12, Ayesha Rehman13
1Deparrtment of Pharmacognosy, Faculty of Pharmacy, Islamia University of Bahawalpur, Bahawalpur. Pakistan
2Department of Pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi, Karachi, Pakistan
3Department of Pharmacy, Benazir Bhutto Shaheed University, Lyari, Karachi, Pakistan
4Department of Basic Medical Sciences, University of Management and Technology,, Lahore, Pakistan
5Department of Botany, Faculty of Science, Islamia University of Bahawalpur, Pakistan
6University college of conventional medicines, Faculty of Pharmacy Islamia University of Bahawalpur, Pakistan
7Department of Pharmaceutics, Faculty of Pharmacy, The Islamia University of Bahawalpur, Pakistan
8Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Islamia University of Bahawalpur, Pakistan
9Islam College of Pharmacy, Sialkot, Pakistan
10Bahawalpur College of Pharmacy, BMDC, Bahawalpur, Pakistan
11Cholistan Institute of Desert Studies, The Islamia University of Bahawalpur, Bahawalpur, Pakistan
12Faculty of Pharmacy, University of Karachi, Karachi Pakistan
13Faculty of Pharmacy Islamia University of Bahawalpur, Bahawalpur, Pakistan
Corresponding Author: [email protected] ; Tel: 0092 3464054083
Recieved Date: 07 April 2021; Accepted Date: 11 August 2021; Published Date: 4 October
Citation: Younus MY, Hasan MH, Hanif M, Sarwar G, Ahmad K, Rehman MS, Shirazi JH, Jamil OA, Khan KR, Syed SK, Ghaffari A, Khan MS, Abdullah M, Wazir MA, Rehman A. Botanical Features and Anti-cancerous Potential of Euphorbia nivulia Buch.-Ham. Trop J Nat Prod Res, 2021; 5(9):1591-1596.
 © 2021 Younus et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Euphorbia nivulia – Buch.-Ham. (En), one of the members of Euphorbiaceae family, is rich in various phytoconstituents including flavonoids and polyphenolics. Many species of the genus Euphorbia have been reported to possess varying biological activities, including anti-proliferatory, cytotoxic, anti-inflammatory and antimicrobial activities. This medicinal plant species indigenous to the Cholistan desert of Bahawalpur, Pakistan, was evaluated for its botanical features and anti-cancerous potential. The species was evaluated for botanical features through anatomical evaluation and powder microscopy. Standard conventional procedures were employed for preliminary qualitative phytochemical screening of En (crude extract) and various fractions, named as En hexane (En hex), En chloroform (En ch), En butanol (En bt) and En aqueous (En aq) to identify various phytoconstituents. Toxicity to Artemia salina (Leach shrimp) larvae was assayed through Brine Shrimp Lethality Assay (BSLA) using Etoposide as standard. Anticancer activity of the plant against 3T3 and HeLa cell lines was assayed using the tetrazolium salt reduction assay. Doxorubicin and Cyclohexamide were used as standard. En showed optimum activity, 73.33% mortality against Artemia salina. Maximum cytotoxic activity against 3T3 and HeLa cells was shown by En and En hex. The current study may be helpful in proper identification of the plant species and suggests that En may be a suitable anticancerous candidate.

Keywords: Euphorbia nivulia, Botanical features, Anti-cancerous potentials, 3T3, HeLa cells.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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