Antioxidant and Lifespan-Extending Effects of a Rejuvenating Thai Traditional Polyherbal Remedy (Phy-Blica-O) in Caenorhabditis elegans

Wipawee Chanthasri1, Goon Jo Aan2, Ngamsiri Singkonpong1, Teerawat Sudkhaw1, Katesarin Maneenoon1, Surasak Limsuwan1, Sineenart Sanpinit3, Palika Wetchakul3, Sasitorn Chusri1,4*

1Faculty of Traditional Thai Medicine, Prince of Songkla University, Hat Yai, Songkhla, 90110 Thailand
2Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur Wilayah Persekutuan, Malaysia
3School of Medicine, Walailak University, 222 Thaiburi, Thasala District, Nakhon Si Thammarat 80160 Thailand
4School of Health Science, Mae Fah Luang University, Thasud, Muang, Chiang Rai, 57100 Thailand

Corresponding Author: [email protected] ; Tel: (+66)53916913
Recieved Date: 15 December 2020; Accepted Date: 2 September 2021; Published Date: 4 October
Citation: Chanthasri W, Aan GJ, Singkonpong N, Sudkhaw T, Maneenoon K, Limsuwan S, Sanpinit S, Wetchakul P, Chusri S. Antioxidant and Lifespan-Extending Effects of A Rejuvenating Thai Traditional Polyherbal Remedy (Phy-Blica-O) in Caenorhabditis elegans. Trop J Nat Prod Res. 2021; 5(9):1554-1568.
© 2021 Chanthasri et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Visit for more related articles at  Tropical Journal of Natural Product Research


Despite the long historical use of polyherbal combinations in traditional medicine, these remedies have only recently gained attention. The purpose of the present study was to explore the antioxidant capacity of traditional tonifying remedies and to examine their effect on the longevity and oxidative stress resistance of Caenorhabditis elegans. Twenty herbal mixture extracts were made as either infusions or decoctions and tested by six different free-radical scavenging techniques. The selected formula, Phy-Blica-O (THP-R016), was used to examine the longevity of C. elegans. The antioxidant-related chemical markers were qualified by HPLC-ESI-MS. Among the tested extracts, Phy-Blica-O possessed remarkable free-radical scavenging activity, with IC50 values of 0.13, 0.2, and 0.07 mg/mL against 1,1-diphenyl-2-picrylhydrazyl free radical, 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonate) radical, and the superoxide anion, respectively, and it had a peroxyl scavenging effect, with an ORAC value of 35.41 µM TE per gram of extract.  Phy-Blica-O had the highest phenolic and flavonoid contents of 651.2 GAE mg/g and 79.4 CAE mg/g, respectively. Phy-Blica-O significantly extended the lifespan of C. elegans under normal growth conditions. It could be attributed to a minor increase in catalase and superoxide dismutase activities, as well as a decrease in lipofuscin and protein carbonyl levels in C. elegans. These results demonstrated the promising antioxidant capacities found in Phy-Blica-O that are partly involved in the longevity of C. elegans. Further analysis of Phy-Blica-O is required to gain insights into the lifespan-extending agents, thereby developing novel, traditional phytochemical interventions for age-related symptoms.

Keywords: Caenorhabditis elegans, Antioxidant activity, Lifespan extending effects, Traditional tonifying remedy.
Back to Articles

ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
Index Copernicus Value (ICV) for 2017: 59.83
Scopus citescore 0.3 (2020)

Indexing & Abstracting

citescore 0.3 (2020)

j-gate logo

International Innovative Journal Impact Factor

African Index Medicus


Index Copernicus International

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License

Crossref Content Registration logo

WorldCat Discovery Service

Geneva Foundation for Medical Education and Research