Antimicrobial and Toxicity Studies on Holisa Herbal Formulation

Abiodun H. Adebayo1*,  Omolara F. Yakubu1, Edvyne S. Ezekiel-Hart1, Olajuwon Okubena2
1Department of Biochemistry, College of Science and Technology, Covenant University Ota, Ogun State, Nigeria

Corresponding Author: [email protected]; Tel: +2348036357197
Recieved Date: 20 March 2021; Accepted Date: 12 May 2021; Published Date: 03 June
Citation: Adebayo AH, Yakubu OF, Ezekiel-Hart ES, Okubena O. Antimicrobial and Toxicity Studies on Holisa Herbal Formulation. Trop J Nat Prod Res. 2021; 5(5):983-987.
© 2021 Adebayo et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Herbal plants have been reported to play crucial role in enhancing human health. The study evaluated the antimicrobial property, acute and sub-chronic toxicological effects of Holisa herbal formulation consisting of Vernonia amygdalina, Rorippa madgasceriensis, and Securinega virosa plants using biochemical and histopathological indices. Thirty (30) male Wistar rats of five groups (A-E) of 6 animals each were used. Animals in group A were given distilled water while those in B, C, D, and E received 20, 40, 60, 100 mL/kg of body weight of the formulation for 28 days. The zone of inhibition was determined with agar well diffusion method using seven bacterial and three fungal isolates. The acute toxicity was carried out with the use of Swiss mice which were observed for 72 hours. The zone of inhibition observed on both the bacterial and fungal culture media was very minimal (6-9 mm). Significant (p<0.05) increase in body weight was observed in groups B, C, and E and a significant (p<0.05) decrease in group D when compared to the control. Alkaline phosphatase, direct bilirubin, triglyceride, total cholesterol, low-density lipoprotein, high-density lipoprotein, creatinine, and total protein were increased significantly (p<0.05), while aspartate aminotransferases, alanine aminotransferases, albumin did not show significant differences. The histopathology conducted showed that the formulation showed congestion in the blood vessels and vascular constriction on the liver and kidney tissues respectively. The results revealed that lower doses of Holisa herbal formulation may not have any adverse effect on the liver and kidney tissues but high doses elicited toxicity.

Keywords: Holisa herbal formulation, Antimicrobial studies, Biochemical studies, Histopathology.
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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