DMBA-Induced Kidney Dysfunction: Effects of Supplementary Dietary Vitamin K in Rats

Oluwatosin A. Dosumu^1*, Adelani I. Bababode², Solomon O. Rotimi², Adio J. Akamo¹, Oluwatosin O. Omotosho¹, Latifah O. Sani¹, Kehinde T. Osinuga¹, Odunayo A. Taiwo^1,3, Oluwafemi P. Owolabi¹, Oluwafemi A. Ojo⁴

¹Department of Biochemistry, Federal University of Agriculture, Abeokuta, Nigeria

²Department of Biochemistry, Covenant University, Sango Ota, Nigeria

³Department of Biochemistry, Chrisland University, Owode, Nigeria

⁴Department of Biochemistry, Landmark University, Omu Aran, Nigeria

Corresponding Author: [email protected] ; Tel: +2347039332134

Recieved Date: 09 November 2020; Accepted Date: 03 May 2021; Published Date: 03 June

Citation: Dosumu OA, Bababode AI, Rotimi SO, Akamo AJ, Omotosho OO,  Sani LO, Osinuga KT, Taiwo OA, Owolabi OP, Ojo OA. DMBA-Induced Kidney Dysfunction: Effects of Supplementary Dietary Vitamin K in Rats. Trop J Nat Prod Res. 2021; 5(5):917-923. http://www.doi.org/10.26538/tjnpr/v5i5.19

Copyright:

© 2021 Dosumu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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ABSTRACT

DMBA (7,12-Dimethyl-1,2-benzathracene) is a polycyclic aromatic hydrocarbon known to cause many toxic effects. Nephrotoxicity occurs following exposure of the kidneys to this potent mutagen and carcinogen (i.e., DMBA). This study investigated the prophylactic potentials of vitamin K-supplemented diet against DMBA-induced nephrotoxicity in experimental rats. Twenty-eight (28) Wistar rats (mean weight of 135 g) were randomly grouped into 4. The control (group 1) was fed with normal rat chow containing the recommended daily allowance (RDA) of vitamin K (0.0075%). Groups 2 and 3 received a single dose of DMBA (80 mg/kg body weight) intragastrically, while group 3 animals were maintained on a surplus vitamin K supplemented diet (0.075%). Group 4 animals were fed the surplus vitamin K supplemented diet alone, with no DMBA challenge. All exposure lasted for 16 weeks. Concentrations of kidney function parameters (BUN and creatinine), pro-inflammatory biomarkers (IL-2 and L-selectin), and electrolytes (K⁺, Na⁺, Mg²⁺, and Ca²⁺) were measured in the serum. Oxidative stress biomarkers (MDA and NO) were also monitored in addition to enzymatic and non-enzymatic antioxidant parameters. Histopathology of the kidney tissues was used to confirm possible alterations and protection. Our investigations revealed significant protection from DMBA-induced renal toxicity by surplus vitamin K inclusion in the diet.