Phenolic Content and Antibacterial Activity of Cupressus sempervirens Leaves Extracts Against Phytopathogenic Bacteria Pectobacterium atrosepticum Causative Agent of Soft Rot on Potato and Clinical Bacteria Strains

Khouloud Ait Ialeff¹, Aicha Debib^2*, Soumaya Menadi¹, Muneer Alsayadi³, Amel Kaced⁴, Atika Benrima¹

¹Laboratory Biotechnology of Plant Productions, Faculty of Nature and Life Sciences, Saad Dahlab University, Blida 1 B.P 270 Soumaa, Blida, Algeria

²Laboratory Management and Valorization of Agricultural & Aquatic Ecosystems, Morsli Abdallah Tipaza University Center, Ouad Merzoug 42000, Algeria

³Department of Food Science and Technology, Ibb University, 70270 Ibb, Yemen 

⁴Center of Scientific and Technical Research in Physico-Chemical analysis (CRAPC), Algeria

Corresponding Author: [email protected]; Tel: +213696577219

Recieved Date: 23 February 2021; Accepted Date: 14 May 2021; Published Date: 03 June

Citation: Ait Ialeff K, Debib A, Menadi S, Alsayadi M, Kaced A, Benrima A. Phenolic Content and Antibacterial Activity of Cupressus sempervirens Leaves Extracts against Phytopathogenic Bacteria Pectobacterium atrosepticum Causative Agent of Soft Rot on Potato and Clinical Bacteria Strains. Trop J Nat Prod Res. 2021; 5(5):866-872. http://www.doi.org/10.26538/tjnpr/v5i5.12

Copyright:

© 2021 Ait Ialeff et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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ABSTRACT

Cupressus sempervirens (Cupressaceae) “cypress” is a specie native to the Mediterranean region. It is a medicinal and an aromatic plant.  This study was carried out to evaluate the antibacterial activity of Algerian Cupressus sempervirens leaves extracts against a phytopathogenic bacteria, Pectobacterium atrosepticum, a causative agent of soft rot on potato Solanum tuberosum, and three clinical bacterial strains (Staphylococcus aureus, Escherichia coli and Klebsiella oxytoca), in both cultured media and on potato tubers. Additionally, the Ethanolic, Acetone, and aqueous extracts were investigated for their phenolic content by using the Folin–Ciocalteu assay. The results revealed that all extracts possessed varying antibacterial activities against the tested Gram-positive and Gram-negative bacteria. Pectobacterium atrosepticum was the most susceptible microorganism to all extracts. Similar result of the antibacterial activity of the extracts, using extracts on potato tubers, was obtained.  A decrease in soft rot development with almost complete inhibition in presence of extracts was observed. The total phenolic content of the investigated extracts varied from 8.30 ± 0.07 mg GAE / g extract to 27.68 ± 1.20 mg GAE / g of extract. The aqueous extract was the richest in total polyphenols (27.68 ± 1.20 mg GAE / g of extract). From the results of this study, it is concluded that the Algerian cypress can be used as an effective natural antibacterial for treating multiple diseases, as well as an excellent biological control against the pathogens of potato rots, and natural food preservative.