Hepatoprotective Effects of Kombucha Tea in Carbon Tetrachloride-Induced Hepatotoxicity: An In Vivo and Molecular Docking Studies

Catherine K.B. Zakhari¹, Samir M. Osman¹, Nadia M. Mahfouz^{2, 3,} Camilia G. Michel⁴, Essam Abdel-Sattar^4*

¹Pharmacognosy Department, Faculty of Pharmacy, October 6 University, Sixth of October City, Egypt 

²Chemistry Department, Faculty of Pharmacy, October 6 University, Sixth of October City, Egypt

Medicinal Chemistry Department, Faculty of Pharmacy, Assiut University, Assiut, Egypt 

⁴Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt

Corresponding Author: [email protected]; Tel: +201065847211

Recieved Date: 18 February 2021; Accepted Date: 07 May 2021; Published Date: 03 June

Citation: Zakhari CKB, Osman SM, Mahfouz NM, Michel CG, Abdel-Sattar E. Hepatoprotective Effects of Kombucha Tea in Carbon Tetrachloride-Induced Hepatotoxicity: An in Vivo and Molecular Docking Studies. Trop J Nat Prod Res, Month 2021; 5(5):857-865. http://www.doi.org/10.26538/tjnpr/v5i5.11

Copyright:

© 2021 Zakhari et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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ABSTRACT

Kombucha is a fermented tea produced by the fermentation of tea with a starter culture known as Symbiotic colony of yeast and bacteria “SCOBY”. This study aimed to determine the best conditions of tea fermentation and to evaluate the antioxidant and hepatoprotective effects in CCl₄-induced hepatotoxicity in rats. This study was performed using CCl₄-induced liver injury in a rat model. The fermented black tea (BTE) and green tea (GTE) extracts at dose level of 100 mg/kg b.wt were administered orally prior to  CCl₄ administration. The antioxidant activity was assessed by the determination of reduced glutathione (GSH) level, while the hepatoprotective effect was assessed by measuring alanine aminotransferase (ALT), aspartate aminotransferase (AST), along with histopathological examination. HPLC investigation was performed for selected polyphenolic compounds and caffeine alkaloid. We found that fermentation of black tea for three weeks gave the best results as indicated by the chemical analysis and by studying the hepatoprotective activity. In vivo antioxidant and hepatoprotective effects revealed a significant decrease in AST, ALT and increase in GSH levels. A molecular docking study was performed regarding the polyphenolic compounds and caffeine, and the results showed a conformational fitting in the active site of human myeloperoxidase [hMPO] (ID: 3ZS0). The results of the current study could be of benefit for the development of functional drink with significant prophylactic effects, particularly in Egypt, which showed high prevalence of liver diseases.