GC-MS Analysis of Essential Oil Composition and Insecticidal Activity of Syzygium aromaticum against Callosobruchus Maculatus of Chickpea

Allali Aimad1*, Rezouki Sanae1, Slim Mostafa1, Mansouri Dalal1, Eloutassi Noureddine2 and Fadli Mohamed1
1Laboratory of Plant, Animal and Agro-industry Productions, Faculty of Sciences, University of Ibn Tofail (ITU), Kenitra Morocco
2Regional Center for the Trades of Education and Training (CRMEF), Fez-Morocco

Corresponding Author: [email protected]; Tel: +2120678673220
Recieved Date: 09 February 2021; Accepted Date: 01 May 2021; Published Date: 03 June
Citation: Aimad A, Sanae R, Mostafa S, Dalal M, Noureddine E, Mohamed F. GC-MS Analysis of Essential Oil Composition and Insecticidal Activity of Syzygium Aromaticum against Callosobruchus Maculatus of Chickpea. Trop J Nat Prod Res. 2021; 5(5):844-849. http://www.doi.org/10.26538/tjnpr/v5i5.9
 © 2021 Aimad et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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The beetle Callosobruchus maculatus (Chrysomelidae: Bruchinae) is a destructive pest of stored chickpea seeds. The use of plant materials rather than conventional pesticides to control these pests is a promising alternative to chemical insecticides. The present study evaluated the insecticidal activity of clove essential oils by three different methods on Callosobruchus maculatus. The oils were obtained by hydro-distillation with a yield of 6.3 ± 0.23% and analyzed by GC-MS. In total, 65 compounds representing 99.92% of the oil were identified and the main components 80.26% Eugenol, 9.62% Eugenyl acetate, 6.74% ?-Caryophyllene and 1.14% C-Humulene. The results of the insecticidal activity of S. aromaticum on C. maculatus performed by inhalation shows that a significant mortality was observed at a concentration of 20 ?l/l against insects exposed at 24 hours (6.67%), 48 hours (36.67%) and 72 hours (100%). Contact test results are lower than those produced by inhalation. Essential oil decreased oviposition and fully suppressed adult growth at the highest dose of 20 ?l/l of air per contact and inhalation test. The results of the present research have shown that EO had a high effectiveness against C. maculatus, and in another hand, these results can be used as a source of natural product to avoid the contamination of chickpea seeds, and also as an alternative to chemical products.

Keywords: Insecticidal activity, Oviposition, Mortality, Contact, Inhalation 
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ISSN: 2616-0684 (Print)
ISSN: 2616-0692 (Online)
DOI: 10.26538/tjnpr
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