Characterization and Anti-Candidal Activity of the Endophytic Streptomyces Isolated from Asystasia gangetica

Duongkamol Phongsopitanun¹, Paranee Sripreechasak², Kanyanat Piewpong³, Wongsakorn Phongsopitanun³, Esthera Prateeptongkum^1*

¹Department of Medical Services, Institute of Dentistry, Nonthaburi 11000, Thailand

²Department of Biotechnology, Faculty of Science, Burapha University, Chonburi 20131, Thailand

³Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand

Corresponding Author: [email protected] ; Tel: +66 2547 0388

Recieved Date: 26 October 2020; Accepted Date: 12 May 2021; Published Date: 03 June

Citation: Phongsopitanun D, Sripreechasak P, Piewpong K, Phongsopitanun W, Prateeptongkum E. Characterization and Anti-Candida Activity of the Endophytic Streptomyces Isolated from Asystasia gangetica. Trop J Nat Prod Res. 2021; 5(5):814-818. http://www.doi.org/10.26538/tjnpr/v5i5.4

Copyright:

© 2021 Phongsopitanun et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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ABSTRACT

Oral candidiasis, or oral thrush, is a common fungal infection of the oral cavity caused by the Candida species. This infection can cause oral discomfort, pain, and loss of appetite. Because of the increase in antifungal resistance among Candida species, alternative antifungal compounds are required to be developed. Actinobacteria, specifically the Streptomyces species, are well known as producers of bioactive compounds and play an important role in drug discovery. The endophytic actinobacteria strain 5R010, isolated from the root of Asystasia gangetica, was identified using phenotypic properties and 16S rRNA gene analysis. The spiral spore chains were observed on the aerial mycelia of strain 5R010. LL-diaminopimelic acid was detected in the whole-cell hydrolysate. The strain showed the closest 16S rRNA gene similarity of 99.72% with Streptomyces sioyaensis as well as sharing the same node with S. sioyaensis in the phylogenetic tree. On the basis of the agar disc diffusion method, the crude ethyl acetate extract of strain 5R010 showed inhibitory activity against seven tested Candida species including Candida albicans TISTR 5554, Candida glabrata TISTR 5006, Candida guilliermondii TISTR 5206, Candida krusei TISTR 5351, Candida parapsilosis TISTR 5007, Candida pseudotropicalis TISTR 5336, and Candida tropicalis TISTR 5268. Based on the results obtained from this study, S. sioyaensis 5R010 can be employed in further research involving the isolation of antifungal agents.