Solanum macrocarpon Leaf Ameliorates Phenylhydrazine-induced Hemolytic Anemia, Hepatotoxicity and Oxidative Stress in Wistar Rats
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Abstract
Several epidemiological studies have reported that the consumption and usage of plant food with bioactive content is associated with reduced incidence of pathologies. This study investigated the protective role of the aqueous leaf extract of Solanum macrocarpon against hemolytic anemia and liver toxicity caused by Phenylhydrazine in rats. The study was comprised of five groups (six albino rats each). Group 1 was normal control orally given normal saline once daily. Group II was an anemic group while Group III was orally given a daily dose of 300mg/kg Solanum macrocarpon aqueous leaf extract for 14 days. Group IV was induced with anemia and thereafter orally treated with 300mg/kg Solanum macrocarpon aqueous leaf extract for 14 days while Group V was induced with anemia and thereafter treated with 100mg/kg Ascorbic acid orally once daily for 14 days. Group II, IV, and V were intraperitoneally once daily induced hemolytic anemia with 50mg/kg Phenylhydrazine for three days consecutively. All rats were sacrificed on day 15. The observed significant reduction (p<0.05) in red blood cell (RBC), hemoglobin (Hb), packed cell volume (PCV), and Platelet count following Phenylhydrazine-induced anemia were attenuated after Solanum macrocarpon administration. Phenylhydrazine-induced anemia also increased significantly (p<0.05) aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), malondialdehyde (MDA), nitric oxide (NO) content with a concomitant decrease in catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase (GPx), all mitigated and reversed toward normalcy by Solanum macrocarpon leaf and Ascorbic acid corroborated by hepatic histopathological analysis. Thus, Solanum macrocarpon comparing favorably with ascorbic acid administration attenuated Phenylhydrazine-induced hemolytic anemia, toxicity, oxidative stress, and inflammation via mechanisms that involved its antioxidant and anti-inflammatory properties.
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1.Gallagher PG. Anemia in the pediatric patient. Blood 2022;140(6):571–593. DOI 10.1182/BLOOD.2020006479.
2.Baldwin C, Pandey J, Olarewaju O. 2022. Hemolytic anemia. Pathobiology of Human Disease: A Dynamic Encyclopedia of Disease Mechanisms 2022;115(6):1532–1543 DOI 10.1016/B978-0-12-386456-7.07907-7.
3.Nawaz H, Muhammad AS, Mohammad SI. Optimization of phenylhydrazine induced hyperbilirubinemia in experimental rabbit. Exp Anim. 2016; 65(4), 363–372.
4.Shukla P, Yadav NK, Singh P, Bansode FW, Singh RK. Phenylhydrazine induced toxicity: A review on its haematotoxicity. Int J. Basic Appl Med. Sci. 2012;2:86–91.
5.Nakanishi A, Kinuta K, Abe T, Araki K, Yoshida Y, Liang S, Li, SA, Takei K, Kinuta M. Formation of meso, N-diphenylprotoporphyrin IX by an aerobic reaction of phenylhydrazine with oxyhemoglobins. Acta Med. Okayama 2003;57:249–256.
6.Atawodi SE. Antioxidant potential of African medicinal plants. Afri J. Biotech. 2005;4:128-133.
7.Bukenya-Ziraba R, Bonsu KO. Solanum macrocarpon L. In: Grubben GJH, Denton OA (eds). Plant Resources of Tropical Africa. Vegetables/Legumes, Wageningen, Netherlands. 2004.
8.Oladiran JA. The effects of fruit color, processing technique and seed treatment on the germination of Solanum macrocarpon L. (Igbagba). Nig J. Tech Res. 1989;1(1):17–20.
9.Edijala JK, Asagba SO, Eriyamremu GE, Atomatofa U. Comparative Effect of Garden Egg Fruit, Oat and Apple on Serum Lipid Profile in Rats Fed a High Cholesterol Diet. Pak J. Nutr. 2005;4(4):245-249. http://dx.doi.org/10.3923/pjn.2005.245. 249.
10.Dalziel JM. The useful plants of West Tropical Africa (New York: Longman) 1st edn. pp. 1937;433–435.
11.Usunobun U, Igwe VC. Phytochemical analysis, mineral composition and in vitro antioxidant activities of Solanum macrocarpon leaves. Int J. Health 2016;4(1): 62 – 65.
12.Aziza AM, El-Shafey MM, El-Ezabi HHM, Ouda MM, Hegazy DSI. Effect of wheat germ oil on phenylhydrazine-induced toxicity in male albino rats. Egypt. J. Exp. Biol. (Zool.), 2023;19(1):51 – 59 DOI : 10.5455/egysebz.20230108094930
13.Chinko BC, Pughikumo DT, Obia O, Udeh WC, Hart VO. Honey Attenuates Phenylhydrazine-Induced Hematotoxicity and Oxidative Stress in Male Wistar Rats. Int Blood Res & Rev. 2023;14(3):10-18.
14.Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95: 351-358. https://doi.org/10.1016/0003-2697(79)90738-3
15.Cohen G, Dembiec D, Marcus, J. Measurement of catalase activity in tissue extracts. Anal Biochem 1970;34:30-38. https://doi.org/10.1016/0003-2697(70)90083-7
16.Misra HP, Fridovich I. The role of superoxide anion in the autooxidation of epinephrine and a simple assay for superoxide dismutase. J. Biol Chem. 1972; 247: 3170-3175. https://doi.org/10.1016/S0021-9258(19)45228-9
17.Ellman GL. SH groups determination in biological fluids,” Anal Biochem, 1970; 46:237–240.
18.Nyman M. Serum hemoglobin; methodological and clinical studies. Scand J. Clin and Lab Invest 1959;11:1-169.
19.Montogomery H, Dymock J. The determination of nitrite in water: colorimetric method of nitric oxide assay. Analyst, 1961;86: 414.
20.Nwachukwu DA, Uchendu IK, Nwafor GO, Ogbonna CC, Kwaor IA, Onyishi JC, Nwokwu OH , Jideofor GC, Okpara OM, Obinwa OR, Simon EO, Ewurum EC, Agbowu IB, Ishaq AO, Kalu PC, Ajibo VC, Ugochukwu IP, Okereke SE , Ojo UO. The Effects of Flauzifop-p-butyl on Behavioural Changes, Acetylcholinesterase, Serum Biochemical Parameters, and Hematological Indices in Albino Rats. Trop J Nat Prod Res. 2024;8(10): 8810 – 8818 https://doi.org/10.26538/tjnpr/v8i10.25
21.Soliman NA, Mansour SW, Ammar MA, Hassan NA, Mohamed RHA. Ameliorative Effect of Pomegranate Molasses on Phenylhydrazine-Induced Anemia in Rats. Trop J Nat Prod Res. 2024; 8(7): 7909 – 7914. https://doi.org/10.26538/tjnpr/v8i7.38
22.Kale OE, Awodele O, Akindele AJ. Protective effects of Acridocarpus smeathmannii (DC.) Guill. & Perr. root extract against phenylhydrazine-induced haematotoxicity, biochemical changes, and oxidative stress in rats. Biochem. Insights, 2019; 12:1178626419883243. doi: 10.1177/1178626419883243.
23.Amama EA, Udefa AL, Beshel FN, Archibong EA, Okpa S, Nwangwa JN, Felix NI, Akronyi G. Fresh palm oil improves impaired renal function in phenylhydrazine-induced anemic Wistar rats via its anti-anemic effect and modulation of expressions of pro-oxidant/antioxidants, inflammatory cytokines and caspase-3 in the kidneys. Phy. Pharma. 2022; 26:299-312. http://dx.doi.org/10.52547/phypha.26.3.8
24.Usunobun U, Inuumoru SC, Onyeaghor C, Lawuru D, Egbo H. Nephroprotective potential of Chromolaena odorata (L.) R.M. King & H.Rob. on Methotrexate-induced Kidney damage and Oxidative stress. J. Res Appl Basic Med Sci. 2022;8(3):118-127
25.Usunobun U, Okugbo OT. Anti-anemic, Anti-necrotic and Anti-fibrotic effect of Vernonia amygdalina post-treatment in Dimethylnitrosamine (DMN)-administered rats. Nig J. Pharma Appl Sci Res. 2016;5(2):1-7.
26.Yuan G, Dai S, Yin Z. Toxicological assessment of combined lead and cadmium: acute and sub-chronic toxicity study in rats. Food and Chem Toxic. 2014;65:260-268.
27.Stevens GA, Bennett JE, Hennocq Q. Trends and mortality effects of vitamin A deficiency in children in 138 low-income and middle-income countries between 1991 and 2013: a pooled analysis of population-based surveys. Lancet Glob Health 2015;3(09):e528–e536
28.Britton RS, Bacon BR. Role of free radicals in liver diseases and hepatic fibrosis. Hepatogastroenterology 1994;41:343-348
29.Pal P, Banerjee ER. Exploring the Effect of Phytochemical Extract (Benincasa sp.) on the Inflammatory Mediators and Regulatory Pathways of Acute Cisplatin Nephrotoxicity in Preclinical Mouse Model. Biomed. Res. Ther. 2024;11(6):6494-6510. DOI : 10.15419/bmrat.v11i6.896
30.Etim NN, Williams ME, Akpabio U, Offiong EE. Hematological parameters and factors affecting their values. J Agric Sci. 2014;2(1):37-47.
31.Airaodion AI, Ogbuagu EO. Consumption of tiger nut (Cyperus esculentus L.) improves hematopoiesis in Wistar rats. Int J. Res Rep in Hema. 2020;3(1):13-19.
32.Hassan HA, Hassan HA. The potential effect of tigernut oil on some haemato-biochemical blood indices in male albino rats. Egypt. J. Exp. Biol.(Zool.), 2007;3:49-54.
33.Usunobun U, Igwe VC. Phytochemical analysis, mineral composition and in vitro antioxidant activities of Solanum macrocarpon leaves. Int J. Health 2016;4(1):62–65.
34.Muriithi NJ, Maina GS, Maina MB, Kiambi MJ, Kelvin JK, Umar A, John MK, Ann NW, Piero NM, Eliud NNM. Determinants of hematological effects of methanolic leaf extract of Vernonia lasiopus in normal mice. J. Blood and Lymph, 2015;5(1):139
35.Chen X, Li B, Gao Y, Ji J, Wu Z, Chen S. Saponins from Sanguisorba officinalis improve hematopoiesis by promoting survival through FAK and Erk1/2 activation and modulating cytokine production in bone marrow. Front Pharmacol. 2017;8:130. doi: 10.3389/fphar.2017.00130.
36.Usunomena U, Okolie NP, Eze IG. Inhibitory Effect of Vernonia amygdalina on Dimethylnitrosamine (DMN)-induced Liver Fibrosis in Rats. Int J Clin Pharmacol Toxicol, 2015;4(4):179-184
37.Ikponmwosa BO, Usunobun U. Aqueous Leaf Extract of Chromolaena odorata Attenuates Methotrexate-Induced Hepatotoxicity in Wistar Rats. J. Fund Appl Pharm Sci. 2022;3(1):16-29.
38.Usunobun U, Osaigbovo JO, Okolie NP. Hepatoprotective Effect of Raphiostylis Beninensis Ethanol Root Extract on Carbon-tetrachloride (CCl4)-induced Liver Attack and Damage in Rats. Am. J. Biomed. Sci. 2020;12(3),155-163; doi:10.5099/aj200300155
39.Usunobun U, Okolie NP. Synergistic, attenuative and modulatory activity in Dimethylnitrosamine (DMN)-induced fibrotic rats treated with Vernonia amygdalina and Annona muricata leaves. Nig J. Pharma Appli Sci Res. 2020;9(3):1-6
40.Kaptanoglu L, Kapan M, Kapan S. Effects of nimodipine and pentoxyfylline in prevention of hepatic ischemic damage in rats at normal and hypothermic conditions. Euro J. Pharma 2008; 587:253-256.
41.Mansourian M, Sadeghi H, Doustimotlagh AH. Activation of the glutathione peroxidase by metformin in the bile-duct ligation induced liver injury: in vivo combined with molecular docking studies. Curr Pharm Des 2018; 24(27):3256–3263.
42.Sharma V, Gupta R, Sharma S. Preventive Effects of Tinospora cordifolia extract against Aflatoxin-b1 induced oxidative stress in Swiss albino mice. Asian J Pharm Clin Res 2011;4(4): 149-155.
43.Gharu CP. Defense mechanism of natural antioxidants against free radicals. CAJMS. 2022; 3:163–170.
44.Bansode FW, Arya KR, Meena AK, Singh RK. Hematinic and anti-hematinic effects of the methanol extract of Saraca
Indica stem bark against phenylhydrazine induced anemia in rats. World J. Pharm. Res., 2019; 8(9):1176-1201.
45.Fibach E, Rachmilewitz E. The role of oxidative stress in hemolytic anemia. Curr Mol Med. 2008;8:609-619
46.Onoja SO, Omeh YN, Ezeja MI, Chukwu MN. Evaluation of the in vitro and in vivo antioxidant potentials of Aframomum melegueta methanolic seed extract. J Trop Med 2014; 2014:159343.
47.Aydin S, Aral I, Kilic N, Bakan I, Aydin S, Erman F. The level of antioxidant enzymes, plasma vitamins C and E in cement plant workers. Clin. Chim. Acta 2004; 341:193–198. https://doi.org/10.1016/j.cccn.2003.12.007
48.Da Pozzo E, De Leo M, Faraone I, Milella L, Cavallini C, Piragine E, Filippi A, Braca A, Martinelli C, Bilia AR, Pistelli L. Antioxidant and antisenescence effects of bergamot juice. Oxid Med Cell Longev. 2018:9395804.
49.Cross RK, Wilson KT. Nitric oxide in inflammatory bowel disease. Inflamm Bowel Dis 2003; 9:179–189.
50.Isuosuo CC, Akaneme FI, Abu NE. Nutritional Evaluation of the Seeds of Corchorus olitorius: A Neglected and Underutilized Species in Nigeria. Pak J Nutr. 2019; 18:692-703.
51.Airaodion AI, Ayita EB, Oluba SO, Emaleku SA, Osunmuyiwa OJ. Chemical composition and nutraceutical potential of velvet tamarind fruit pulp. Asian J Biochem. Gene. Mol. Biol. 2021;3(5):22-31.
52.Balogun IO, Olatidoye OP. Chemical composition and nutritional evaluation of velvet bean seeds (Mucuna utilis) for domestic consumption and industrial utilization in Nigeria. Pak. J. Nutr., 2012; 11:116-122.