Effect of Heating Temperatures on the Physicochemical Parameters and Antihyperglycemic Activity of Argan seeds oil In vitro and In vivo
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Abstract
Argania spinosa seed oil is traditionally used in Morocco for diabetes management due to its antihyperglycemic properties. Although, the optimal roasting temperature for seed preparation to maximize oil quality while preserving bioactive compounds remains unexplored. This study aimed to identify the best roasting temperature that maintains the oil’s antihyperglycemic efficacy and physicochemical integrity. Argan seeds were roasted at temperatures ranging from 0 to 150 °C, and the resulting oils were subjected to both in vitro and in vivo evaluation. Roasting increased oil yield and polyphenol content, which peaked at 8.46 ± 0.02 µg GA/mg at 100 °C; above this temperature, polyphenol levels declined. Temperatures above 100 °C adversely affected peroxide value, free acidity, α-amylase inhibitory activity, and hemoglobin antiglycation activity. Furthermore, roasting above 75 °C significantly reduced free-radical-scavenging activity and tocopherol content, while roasting above 125 °C impaired antioxidant activity (β-carotene bleaching test), tannin content, and carotenoid levels. Fatty acid and flavonoid contents remained stable across all temperatures. These findings suggest that roasting Argan seeds at approximately 100 °C produces oil of optimal quality with preserving antihyperglycemic properties.
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1 Azizi S, Mzabri I, Dalli M, Koddane N, Berrichi A, Gseyra N. Eastern Morocco Argania spinosa propagation and growth: A follow-up study. Aust J Crop Sci 2022; 16: 612–619.
2 Zaroual H, Boughattas F, Karoui R. Traditional Foods in Maghreb: Production and Research Progress. In: Al-Khusaibi M, Al-Habsi N, Shafiur Rahman M (eds). Traditional Foods: History, Preparation, Processing and Safety. Springer Inter Publishing: Cham, 2019, pp 51–113.
3 Abdalla S, Aroua MK, Gew LT. A Comprehensive review of plant-based cosmetic oils (Virgin coconut oil, olive oil, argan oil, and jojoba oil): Chemical and biological properties and their cosmeceutical applications. ACS Omega 2024; 9: 44019–44032.
4 Charrouf Z, Guillaume D, Driouich A. The argan tree, an asset for Morocco. Biofutur 2002; 220: 54–57.
5 Drissi A, Girona J, Cherki M, Godàs G, Derouiche A, Messal ME, Saile R, Kettani A, Solà R, Masana L, Adlouni A. Evidence of hypolipemiant and antioxidant properties of argan oil derived from the argan tree (Argania spinosa). Clin Nutr 2004; 23: 1159–1166.
6 Berrougui H, Alvarez de Sotomayor M, Pérez-Guerrero C, Ettaib A, Hmamouchi M, Marhuenda E, Marhuenda E, Dolores Herrera M. Argan (Argania spinosa) oil lowers blood pressure and improves endothelial dysfunction in spontaneously hypertensive rats. Br J Nutr 2004; 92: 921–929.
7 Rabbaa S, Bouchab H, Laaziouez Y, Limami Y, Nasser B, Andreoletti P, Cherkaoui-Maleki M, El Kebbaj R. Argan oil: A natural bioactive lipid modulating oxidative stress and inflammation. Antioxidants 2025; 14: 515.
8 Adlouni A, Christon R, Cherki M, Khalil A, ElMessal M. The nutritional benefits of Argan oil in obesity risk prevention. Atheroscler Suppl 2008; 9: 137–138.
9 Faddladdeen KA, Alotaibi HM. Histological Investigation of the Beneficial Effects of Argan Oil and Statins on the Testis and Epididymis of Obese Rats. J Microsc Ultrastruct 2024. 10.4103/jmau.jmau_193_23.
10 Mekhfi H, Belmekki F, Ziyyat A, Legssyer A, Bnouham M, Aziz M. Antithrombotic activity of argan oil: An in vivo experimental study. Nutrition 2012; 28: 937–941.
11 Khallouki F, Younos C, Soulimani R, Oster T, Charrouf Z, Spiegelhalder B, Bartsch H, Owen RW. Consumption of argan oil (Morocco) with its unique profile of fatty acids, tocopherols, squalene, sterols and phenolic compounds should confer valuable cancer chemopreventive effects. Eur J Cancer Prev 2003; 12: 67–75.
12 Mekhfi H, Gadi D, Bnouham M, Ziyyat A, Legssyer A, Aziz M. Effect of argan oil on platelet aggregation and bleeding time: A beneficial nutritional property. J Complement Integr Med 2008; 5. doi:10.2202/1553-3840.1164.
13 Bellahcen S, Mekhfi H, Ziyyat A, Legssyer A, Hakkou A, Aziz M, Bnouham M. Prevention of chemically induced diabetes mellitus in experimental animals by virgin argan oil. Phytother Res 2012; 26: 180–185.
14 Bnouham M, Bellahcen S, Benalla W, Legssyer A, Ziyyat A, Mekhfi H. Antidiabetic activity assessment of Argania spinosa oil. J Complement Integr Med 2008; 5. doi:https://doi.org/10.2202/1553-3840.1180.
15 Bouyahya A, El Omari N, Elmenyiy N, Guaouguaou F-E, Balahbib A, Belmehdi O, Salhi N, Imtara H, Naceiri Mrabti H, El-Shazly M, Bakri Y. Moroccan antidiabetic medicinal plants: Ethnobotanical studies, phytochemical bioactive compounds, preclinical investigations, toxicological validations and clinical evidences; challenges, guidance and perspectives for future management of diabetes worldwide. Trends Food Sci Technol 2021; 115: 147–254.
16 Daoudi NE, El Assri S, Aziz M, Choukri M, Mekhfi H, Legssyer A, Ziyyat A, Bnouham M.The effect of Argania spinosa seed oil on diabetic nephropathy in streptozotocin-induced diabetes in Wistar rats. J Ethnopharmacol 2024; : 118083.
17 Daoudi NE, Bouhrim M, Ouassou H, Legssyer A, Mekhfi H, Ziyyat A, Aziz M, Bnouham M. Inhibitory effect of roasted/ unroasted Argania spinosa seeds oil on α- glucosidase, α-amylase and intestinal glucose absorption activities. S Afric J Botany 2020; 135: 413–420.
18 Melhaoui R, Kodad S, Houmy N, Belhaj K, Mansouri F, Abid M, Addi M, Mihamou A, Serghini Caid H, Elamrani A. Chemical characterization of local “Beldi” almond oil for food and cosmetic purposes in northeastern of Morocco. E3S Web Conf 2022; 337: 04005.
19 Folin O, Ciocalteu V. On tyrosine and tryptophane determinations in proteins. J Biol Chem 1927; 73: 627–650.
20 Kim D, Chun O, Kim Y, Moon H, Lee C. Quantification of polyphenolics and their antioxidant capacity in fresh plums. J Agric Food Chem 2003; 51: 6509–6515.
21 Julkunen-Tiitto R. Phenolic constituents in the leaves of northern willows: methods for the analysis of certain phenolics. J Agric Food Chem 1985; 33: 213–217.
22 Minguez‐Mosquera MI, Gandul‐Rojas B, Garrido‐Fernandez J, Gallardo‐Guerrero L. Pigments present in virgin olive oil. J Am Oil Chem’ Soc 1990; 67: 192–196.
23 Wotto D V, N’dayishimiye V, Yété P, Sessou P, Agbangnan P, Sohounhloué D. Physico-chemical characterization of oil and defatted meal from Anacardium occidentale acclimated to teval in Northen Benin. World J Pharm Pharm Sci 2015; 4: 1912–1920.
24 Houmy N, Eseghir L, Fethi M, Elfazazi K, Noutfia Y, Melhaoui R, Zerouali EH, Elamrani A. Evaluation of the quality and chemical composition of three oils extracted from citrus seeds grown in easternMorocco. Afric Mediter Agric J - Al Awamia 2022; : 292–305.
25 Taaifi Y, Benmoumen A, Belhaj K, Aazza S, Abid M, Azeroual E, Elamrani A, Mansouri F, Serghini Caid H. Seed composition of non-industrial hemp (Cannabis sativa L.) varieties from four regions in northern Morocco. Inter J Food Sci Technol 2021; 56: 5931–5947.
26 Sánchez-Moreno C, Larrauri JA, Saura-Calixto F. A procedure to measure the antiradical efficiency of polyphenols. J Sci Food Agric 1998; 76: 270–276.
27 Daoudi NE, Bouhrim M, Bouziane O, Abdnim R, Bouknana S, Elrherabi A, Mekhfi H, Aziz M, Legsseyer A, Ziyyat A, Bnouham M. Acute and subchronic treatment of roasted and unroasted argan oil on postprandial glycemia and its effect on glucose uptake by isolated rat hemidiaphragm. Lett Drug Des Discov 2023; 20: 1821–1829.
28 Amanchar M, Harit T, Cherfi M, Idrissi Yahyaoui M, Daoudi NE, Yahyi A, Asehraou A, Malek F. New generation of hybrid pyrazole–tetrazole tetrapodal compounds: Synthesis and biological activities. Organics 2024; 5: 290–297.
29 National Institutes of Health. Public Health Service Policy on Humane Care and Use of Laboratory Animals. 2015; : 1–22.
30 Mamri S, Daoudi NE, Baraich A, Ouahhoud S, Khoulati A, Choukri M, Asehraou A, Bnouham M, Jaouadi B, Abousalham A, Dabielil F, Salamatullah AM, Saalaoui E. Comprehensive analysis of bioactive compounds in Crocus sativus stamens extracts by HPLC-DAD: investigating antidiabetic activity via in vitro, in vivo, and molecular docking simulation. Front Chem 2024; 12. doi:10.3389/fchem.2024.1419120.
31 Karrar E, Mohamed Ahmed IA, Manzoor MF, AL-Farga A, Wei W, Albakry Z, Albakry Z, Sarpong F, Wang X. Effect of roasting pretreatment on fatty acids, oxidative stability, tocopherols, and antioxidant activity of gurum seeds oil. Biocatal Agric Biotechnol 2021; 34: 102022.
32 Cai Z, Li K, Lee WJ, Reaney MTJ, Zhang N, Wang Y. Recent progress in the thermal treatment of oil seeds and oil oxidative stability: A review. Fundam Res 2021; 1: 767–784.
33 Guillaume D, Charrouf Z. Argan oil and other argan products: Use in dermocosmetology. Eur J Lipid Sci Tech 2011; 113: 403–408.
34 El Abbassi A, Khalid N, Zbakh H, Ahmad A. Physicochemical characteristics, nutritional properties, and health benefits of argan oil: A review. Crit Rev Food Sci Nutr 2014; 54: 1401–1414.
35 Matthäus B, Ozcan MM. Quantitation of fatty acids, sterols, and tocopherols in turpentine (Pistacia terebinthus Chia) growing wild in Turkey. J Agric Food Chem 2006; 54: 7667–7671.
36 Matthäus B, Özcan MM. Habitat effects on yield, fatty acid composition and tocopherol contents of prickly pear (Opuntia ficus-indica L.) seed oils. Sci Hortic 2011; 131: 95–98.
37 Durmaz G, Gökmen V. Impacts of roasting oily seeds and nuts on their extracted oils. Lipid Technol 2010; 22: 179–182.
38 Borchani C, Besbes S, Blecker C, Attia H. Chemical characteristics and oxidative stability of sesame seed, sesame paste, and olive oils. J Agric Sci Technol 2010; 12: 585–596.
39 Codex Alimentarius. Standard for vegetable oils bearing a specific name (CODEX STAN 210- 1999), https://www.fao.org/fao-who- codexalimentarius/, 2013.
40 Melhaoui R, Kodad S, Houmy N, Belhaj K, Mansouri F, Abid M, Addi M, Mihamou A, Sindic M, Serghini-Caid H, Elamrani A. Characterization of sweet almond oil content of four european cultivars (ferragnes, ferraduel, fournat, and marcona) recently introduced in Morocco. Scientifica 2021; 2021: e9141695.
41 Flores A, Camacho D. Thermal degradation of coconut oil with free fatty acid, peroxide value, and moisture content indicators. KIMIKA 2023; 34: 49–60.
42 Pazdro R, Burgess JR. The role of vitamin E and oxidative stress in diabetes complications. Mech Ageing Dev 2010; 131: 276–286.
43 Lordan S, Smyth TJ, Soler-Vila A, Stanton C, Ross RP. The α-amylase and α-glucosidase inhibitory effects of Irish seaweed extracts. Food Chem 2013; 141: 2170–2176.
44 Kumari M, Jain S. Tannins: An antinutrient with positive effect to manage diabetes. Res J Recent Sci 2012; 12: 1–8.
45 Sayahi M, Shirali S. The antidiabetic and antioxidant effects of carotenoids: A Review. Asian J Pharm Res Health Care 2017; 9: 186–191.
46 Zhang Y, Oguzhan B, Louchami K, Chardigny J-M, Portois L, Carpentier Y, Malaisse WJ, Herchuelz A, Sener A. Pancreatic islet function in omega-3 fatty acid-depleted rats: alteration of calcium fluxes and calcium-dependent insulin release. Am J Physiol Endocrinol Metab 2006; 291: E441-448.
47 Sawada K, Kawabata K, Yamashita T, Kawasaki K, Yamamoto N, Ashida H. Ameliorative effects of polyunsaturated fatty acids against palmitic acid-induced insulin resistance in L6 skeletal muscle cells. Lipids Health Dis 2012; 11: 36.
48 Situmorang PC, Zuhra CF, Lutfia A, Pasaribu KM, Hardiyanti R, Nugraha AP. Harnessing phytochemicals to combat diabetes: Insights into molecular pathways and therapeutic advances. J Funct Foods 2025; 128: 106799.
49 Daoudi NE, Mamri S, Marghich M, Aziz M, Mekhfi H, Saalaoui E, Choukri M, Legsseyer A, Ziyyat A, Bnouham M. Comparative analysis of nephroprotective effects of roasted and unroasted argan seed oils against gentamicin-induced nephrotoxicity in Wistar rats. S Afric J Bot 2025; 177: 100–108.
50 Belcadi-Haloui R, Zekhnini A, El-Alem Y, Hatimi A. Effects of roasting temperature and time on the chemical composition of argan oil. Int J Food Sci 2018; 2018: 7683041.
51 Bakour M, Soulo N, Hammas N, Fatemi HE, Aboulghazi A, Taroq A, Abdellaoui A, Al-Waili N, Lyoussi B. The antioxidant content and protective effect of argan oil and Syzygium aromaticum essential oil in hydrogen peroxide-induced biochemical and histological changes. Int J Mol Sci 2018; 19: 610.
52 Cherki M, Derouiche A, Drissi A, El Messal M, Bamou Y, Idrissi-Ouadghiri A, Khalil A, Adlouni A. Consumption of argan oil may have an antiatherogenic effect by improving paraoxonase activities and antioxidant status: Intervention study in healthy men. Nutr, Metab Cardiovasc Dis 2005; 15: 352–360.
53 Umadevi S, Gopi V, Vellaichamy E. Inhibitory effect of gallic acid on advanced glycation end products induced up-regulation of inflammatory cytokines and matrix proteins in H9C2 (2-1) cells. Cardiovasc toxicol 2013; 13. doi:10.1007/s12012-013-9222-2.
54 Daoudi NE, Bouziane O, Bouhrim M, Bnouham M. Natural aldose reductase inhibitors for treatment and prevention of diabetic cataract: A review. Herba Pol 2022; 68: 35–58.
55 Bruscatto MH, Pestana-Bauer VR, Otero DM, Zambiazi RC. Effects of heating temperature on the tocopherol contents of chemically and physically refined rice bran oil. Grasas Aceites 2019; 70: e294–e294.
56 Martinez PFM. Studies and experiments on the concentration of tocopherols and phytosterols through molecular distillation. 2006.
57 Figueiredo-González M, Grosso C, Valentão P, Andrade PB. α-Glucosidase and α-amylase inhibitors from Myrcia spp.: a stronger alternative to acarbose? J Pharm Biomed Anal 2016; 118: 322–327.
58 Nakamura Y, Ogawa M, Nishide T, Emi M, Kosaki G, Himeno S, Matsubara K. Sequences of cDNAs for human salivary and pancreatic α-amylases. Gene 1984; 28: 263–270.
59 Brayer GD, Luo Y, Withers SG. The structure of human pancreatic alpha-amylase at 1.8 A resolution and comparisons with related enzymes. Protein Sci 1995; 4: 1730–1742.
60 Mohd Dom NS, Yahaya N, Adam Z, Nik Abd. Rahman NMA, Hamid M. Antiglycation and antioxidant properties of Ficus deltoidea varieties. Evid Based Complementary Altern Med 2020; 2020: e6374632.
61 Dalli M, Daoudi NE, Abrigach F, Azizi S, Bnouham M, Kim B, Gseyra N. In vitro α-amylase and hemoglobin glycation inhibitory potential of Nigella sativa essential oil, and molecular docking studies of its principal components. Front Pharmacol 2022; 13.
62 Daoudi NE, Abdnim R, Elkhadraoui G, Mekhfi H, Legssyer A, Ziyyat A, Bnouham M. Investigation of in vitro antioxidant and hemoglobin antiglycation effect of roasted and unroasted Argania spinosa L. seed oil from Morocco. Curr Chem Biol 2023; 17: 182–190.
63 Marghich M, Daoudi NE, Amrani O, Addi M, Hano C, Chen J-T, Mekhfi H, Ziyyat A, Bnouham M, Aziz M. Antioxidant activity and inhibition of carbohydrate digestive enzymes activities of Artemisia campestris L. Front Biosci-Schol 2022; 14: 25.