Potential of Cucumis sativus L as an Anti-hyperuricemic Agent in Male Rats
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Abstract
Uric acid is a byproduct of purine metabolism. Excessive accumulation of uric acid in joint tissues results in gout, which is characterized by inflammation and pain due to the deposition of uric acid crystals in the joints. Xanthine oxidase, a key enzyme responsible for the production of uric acid, is the main target in gout treatment. Cucumber (Cucumis sativus L.) is rich in flavonoids, which are known to inhibit xanthine oxidase, potentially reducing uric acid concentration. This study aimed to assess the uric acid reducing ability of cucumber juice in rats. A total of 25 male Wistar rats were divided into five groups: a negative control group, which received Na Carboxymethyl Cellulose (0.5%), a positive control group, which received allopurinol (10 mg/kg BW), and three treatment groups (I, II, and III), which received cucumber juice at doses of 0.14 g/200 g, 0.28 g/200 g, and 0.56 g/200 g BW, respectively. Hyperuricemia was induced in the rats by the administration of chicken liver homogenate (5 g/kg) and potassium oxonate (250 mg/kg). The rats were treated for 15 days after hyperuricemia induction according to their grouping. Blood uric acid concentration was measured on days 0, 5, 10, and 15. The results showed that cucumber juice significantly reduced blood uric acid concentration in hyperuricemic rats, with 0.56 g/200 g BW dose exhibiting the highest percentage reduction of 49.8% after 15 days. This suggests that cucumber juice has the potential as a natural alternative that could be used in the treatment of hyperuricemia.
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References
1. So A and Martinon F. Inflammation in Gout: Mechanisms and Therapeutic Targets. Nat Rev Rheumatol. 2017; 13(11):639–647. doi: 10.1038/nrrheum.2017.150.
2. Dalbeth N, Merriman TR, Stamp LK. Gout. Lancet. 2016; 388(10055):2039–2052. doi: 10.1016/S0140-6736(16)00346-9.
3. Kuo CF, Grainge MJ, Zhang W, Doherty M. Global Epidemiology of Gout: Prevalence, Incidence, and Risk Factors. Nat Rev Rheumatol. 2015; 11(11):649-662. doi: 10.1038/nrrheum.2015.91
4. Maiuolo J, Oppedisano F, Gratteri S, Muscoli C, Mollace V. Regulation of Uric Acid Metabolism and Excretion. Int J Cardiol. 2016; 213:8–14. doi: 10.1016/j.ijcard.2015.08.109.
5. Stamp LK, Day RO, Yun J, Kannangara DR. Allopurinol Hypersensitivity: Investigating the Cause and Minimizing the Risk. Nat Rev Rheumatol. 2016; 12(4):235-242. Doi: 10.1038/nrrheum.2016.20
6. Singh B, Singh JP, Kaur A, Singh N. Phenolic Composition and Antioxidant Potential of Cucumis sativus L. Seed Coat and Fruit. J Food Sci Technol. 2019; 56(5):2489–2497. doi: 10.1007/s13197-019-03736-2.
7. Raja MM, Gajalakshmi R, Jayachandran A, Jeyalakshmi K. In Vitro Xanthine Oxidase Inhibitory and Antioxidant Potential of Selected Fruits and Vegetables. Int J Pharm Pharm Sci. 2017; 9(3):133–138. doi: 10.22159/ijpps.2017v9i3.16607.
8. Kim SY, Guevara JP, Kim KM, Choi HK, Heitjan DF, Albert DA. Hyperuricemia and Coronary Heart Disease: A Systematic Review and Meta-Analysis. Arthritis Care Res (Hoboken). 2010; 62(2):170–180. doi: 10.1002/acr.20065.
9. Pangala GB, Mahendra AN, Jawi IM, Dewi NWS. Ethanol Extract of Black Garlic (Allium sativum L.) Reduces Uric Acid Levels in Male Mice Hyperuricemia Model. J Med Uday. 2022; 11(12):89–93. doi: 10.24843/MU.2022.V11.i8.P17.
10. Wang F, Zhao X, Su X, Song D, Zou F, Fang L. Isorhamnetin, the Xanthine Oxidase Inhibitor from Sophora japonica, Ameliorates Uric Acid Levels and Renal Function in Hyperuricemic Mice. Food Funct. 2021; 12(24):12503–12512. doi: 10.1039/d1fo02719k.
11. Zhang G, Zhu M, Liao Y, Gong D, Hu X. Action Mechanisms of Two Key Xanthine Oxidase Inhibitors in Tea Polyphenols and their Combined Effect with Allopurinol. J Sci Food Agric. 2022; 102(15):7195-7208. doi:10.1002/jsfa.12085
12. Sutton SC, Rinaldi MT, Vukovinsky KE. Comparison of the Gravimetric, Phenol Red, and 14C-PEG-3350 Methods to Determine Water Absorption in the Rat Single-Pass Intestinal Perfusion Model. AAPS PharmSci. 2001; 3(3):E25. doi: 10.1208/ps030325.
13. Chua LS, Segaran A, Wong HJ. Identification of Phytochemicals from the Water Extract of Eurycoma longifolia Roots using Solid-Liquid and Liquid-Liquid Extraction Based Fractionation Techniques. Acta Chim Slov. 2021; 68(4):765-772. doi:10.17344/acsi.2020.6154
14. Li G, Zhang Y, Cheng Y, Chao H, Yang X, Dong YM, Li X, Xue H, Wang M, Qi L, Liu J. Metabolomics Reveal the Anti- Hyperuricemia Effects and Mechanisms of Sunflower Head Extract in a Hyperuricemia Mice Model. Mol Nutr Food Res. 2025; 69(3):e2200429. doi: 10.1002/mnfr.202401017.
15. Montgomery, DC. Design and Analysis of Experiments. 10th ed. Hoboken: Wiley; 2020.
16. Francis M, Cull E, Leclair G. Stability of Allopurinol in Extemporaneously Compounded Oral Suspensions with Oral Mix and Oral Mix SF. Int J Pharm Compd. 2024; 28(5):408-412.
17. Silva JGD, Júnior Aires AL, Cunha RXD, Monte TVSD, Assis SPO, Oliveira RN, Souza TGDS, Chagas CA, Silva NJDC, Araújo HDA, Lima VLM. Anti-Hyperuricemic, Anti-Arthritic, Hemolytic Activity and Therapeutic Safety of Glycoconjugated Triazole-Phthalimides. Biomedicines. 2023; 11(9):2537.
18. Wang J, Chen R, Wu K, Mo J, Li M, Chen Z, Wang G, Zhou P, Lan T. Establishment and Optimization of a Novel Rats Model of Hyperuricemic Nephropathy. Ren Fail. 2024; 46(2):2427181. doi:10.1080/0886022X.2024.2427181
19. Ministry of Health of the Republic of Indonesia. General Standard Parameters of Medicinal Plant Extracts. Edition I. Food and Drug Administration. Jakarta. 2000;13-31.
20. Maryam F, Taebe B, Toding DP. Pengukuran Parameter Spesifik Dan Non Spesifik Ekstrak Etanol Daun Matoa (Pometia pinnata J.R & G.Forst). J Man Pharm Ind. 2020; 6(01:1–12. Doi:10.35311/jmpi.v6i01.39
21. Hussain MS, Jahan N, Or Rashid MM, Hossain MS, Chen U, Rahman N. Antihyperlipidemic Screening and Plasma Uric Acid-Reducing Potential of Momordica charantia Seeds on Swiss Albino Mice Model. Heliyon. 2019; 5(5):e01739. Doi: 10.1016/j.heliyon.2019.e01739.
22. Ulya T, Rohmatillah S, Muslichah S, Ningsih IY. Comparison of Antihyperuricemia Activity of Ethanol Extract of Juwet Leaf and Fruit (Syzygium cumini (L.) SKEEL) in Hyperuricemia Mice. J Kef Akf. 2023; 37-43. Doi:10.37089/jofar.v8i1.202.
23. Lewis SE, Li L, Fazzari M, Salvatore SR, Li J, Hileman EA, Maxwell BA, Schopfer FJ, Arteel GE, Khoo NKH, Kelley EE. Obese Female Mice do not Exhibit Overt Hyperuricemia Despite Hepatic Steatosis and Impaired Glucose Tolerance. Adv Redox Res. 2022; 6:100051. Doi:10.1016/j.arres.2022.100051
24. Ban Z, Sun M, Ji H, Ning Q, Cheng C, Shi T, He M, Chen X, Lu H, He X, Guo C, He Y, Shao D, He Y. Immunogenicity-Masking Delivery of Uricase Against Hyperuricemia and Gout. J Contr Rel. 2024; 372:862-873. Doi:10.1016/j.jconrel.2024.06.042
25. Hongwei L, Zhenhai S, Wei J, Bing J, Shaolei L, Ping Z, Liuyu W, Peng Y, Yun L. The Effects and Predictive Values of Novel Anthropometric Parameters on Uric Acid Levels and Hyperuricemia in Adults. Sci Rep. 2025; 15(1):956. Doi: 10.1038/s41598-024-84617-4.
26. Mao Y, Xu H, Xia P. Ilex cornuta Leaf Extracts Ameliorate Hyperuricemia by Modulating Uric acid Transporters. J Ethnopharmacol. 2025; 336:118618. doi:10.1016/j.jep.2024.118618.