Evaluation of UV-Absorbing Capacity, Antioxidant and Antibacterial Activities of Natural Dyes Used in Indonesian Traditional Batik
Article Sidebar
Main Article Content
Abstract
Batik has been part of Indonesian culture, where natural dyes are applied in textile production. The current study aimed to evaluate the health benefits of commonly used natural dyes of Batik; Secang (Caesalpinia sappan L.), Telang (Clitoria ternatea L.) and Putri malu (Mimosa pudica L.) based on their UV absorbing properties, antioxidant and antibacterial activity. UV absorbing properties was evaluated based on UV spectral data analysis. Antioxidant capacity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, while the antibacterial activity was evaluated against against Staphylococcus aureus using the disc diffusion assay. Secang was subjected to gas chromatography-mass spectrometry (GC-MS) analysis to identify potentially bioactive compounds. The UV spectral data analysis indicated that Secang dye absorbs dangerous UV-B radiation at 280 - 315 nm and possesses the highest antioxidant activities with an IC50 value of 10.76 µg/mL. In addition, Secang dye exhibited antibacterial activity against Staphylococcus aureus with inhibition zone diameter of 19.45 ± 1.05 mm at a concentration of 30 mg/mL. GC-MS analysis identified 35 compounds in Secang, in which several common antibacterial active compounds, including glycol congener pentaethylene glycol, pyridine, and naphthalene were present. In summary, natural Batik dyes are highly valued not only for their artistic value, but also for their potential health benefits.
Downloads
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
1. Henar GK, Oderinde O, Wesenhagen G, James NA, Sawirjo F, Ayeni DA, Ogundiran OA. Methods for Quantification of Tannins and Other Polyphenols in Syzygium cumini (L.) Bark for Potential Use in Leather Tanning Colouration. Trop J Nat Prod Res. 2024; 8(11):9086 – 9096.
2. Saensouk P and Saensouk S. Biological Resource of Family Commelinaceae in Maha Sarakham Province: Diversity, Traditional Uses and Conservation Status. Trop J Nat Prod Res. 2023; 7(10):4171-4181.
3. Hidayat J and Fatmahwaty F. The Art and Sustainable Aspects of Natural Dyeing in Kanawida Hand Drawn Batik (Green Batik). IPTEK J Proc Ser. 2014;1(1):136-143.
4. Tereucan G, Ercoli S, Cornejo P, Winterhalter P, Contreras B, Ruiz A. Stability of Antioxidant Compounds and Activities of a Natural Dye From Coloured-Flesh Potatoes in Dairy Foods. LWT Food Sci Technol. 2021; 144:111252.
5. Benli H and Bahtiyari M. Providing UV Protection Features For Woolen Fabric Using Buckthorn Dye. J Nat Fibers. 2023; 20(1):2143978.
6. Waterman MJ, Nugraha AS, Hendra R, Ball GE, Robinson SA, Keller PA. Antarctic Moss Biflavonoids Show High Antioxidant and Ultraviolet-Screening Activity. J Nat Prod. 2017; 80(8):2224-2231.
7. Okafor CE, Ijoma IK, Igboamalu CA, Ezebalu CE, Eze CF, Osita-Chikeze JC, Uzor CE, Ekwuekwe AL. Secondary Metabolites, Spectra Characterization, and Antioxidant Correlation Analysis of the Polar and Nonpolar Extracts of Bryophyllum pinnatum (Lam) Oken. Biotechnol. 2024; 105(2):121-136.
8. Supriyadi A, Arum LS, Nugraha AS, Ratnadewi AAI, Siswoyo TA. Revealing Antioxidant and Antidiabetic Potency of Melinjo (Gnetum gnemon) Seed Protein Hydrolysate at Different Stages of Seed Maturation. Curr Res Nutr Food Sci. 2019; 7(2):479-487.
9. Pamungkas FBP, Triatmoko B, Nugraha AS. Isolasi Fungi Tanah Kabupaten Situbondo Serta Skrining Aktivitas Antibakteri Terhadap Pseudomonas aeruginosa. Indonesian J Pharm Sci. 2021; 19(1):73-79.
10. Inayati A, Qurata Aini L, Yusnawan E. Growth Performance and Metabolic Changes in Susceptible Mung Bean [Vigna radiata (L.) Wilczek] During Interaction with Rhizoctonia solani and Trichoderma virens. Legume Res. 2022; 46(2):228-232.
11. Laaraj S, Ouahidi I, Aarab L. Photoabsorbent, Antioxidative, and Anti-Inflammatory Properties of Three Traditional Cosmetic Seed Oils. Trop J Nat Prod Res. 2022; 6(11):1790-1793.
12. Machado TB, Pinto AV, Pinto MCFR, Leal ICR, Silva MG, Amaral ACF, Kuster RM, Netto-dosSantos KR. In Vitro Activity Of Brazilian Medicinal Plants, Naturally Occurring Naphthoquinones and Their Analogues, Against Methicillin-Resistant Staphylococcus aureus. Int J Antimicrob Agents. 2003; 21(3):279-284.
13. Singh R, Jain A, Panwar S, Gupta D, Khare SK. Antimicrobial Activity of Some Natural Dyes. Dyes Pigm. 2005; 66(2):99-102.
14. Nalawade TM, Bhat K, Sogi SH. Bactericidal Activity of Propylene Glycol, Glycerine, Polyethylene Glycol 400, and Polyethylene Glycol 1000 Against Selected Microorganisms. J Int Soc Prev Commun Dent. 2015; 5(2):114-119.
15. Nanjundaswamy S, Jayashankar J, Renganathan RRA, Karthik CS, Mallesha L, Mallu P, Rai VR. Pyridine Coupled Pyrazole Analogues as Lethal Weapon Against MRSA: An In-Vitro and In-Silico Approach. Microb Pathog. 2022; 166:105508.
16. Sari S, Glu SS, Aslan EK, Avci A, Karta D, Ozdemir Z, Acar MF, Sayoglu B, Alagoz MA, Karakurt A, Dalkara S. Azoles Containing Naphthalene with Activity Against Gram-Positive Bacteria: In Vitro Studies and In Silico Predictions for Flavohemoglobin Inhibition. J Biomol Struct Dyn. 2022; 40(20):10220-10229.