Estrogenic and Antiestrogenic Effects of Methanol Extract of Persea americana Pulp on the Uterus of Immature Female Albino Wistar Rat
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Abstract
Estrogen is important for maturation and maintenance of female reproductive health. The aim is to investigate the estrogenic and antiestrogenic effect of Persea americana pulp on immature female Wistar rats using ovariectomized animals. Exactly 45 immature female rats randomly divided into 9 groups of five animals each were used. Ovariectomy was done for 40 rats. Group 1 served as the control, group 2 received distilled water as the sham operated group, group 3 received 0.1mg/kg of 17ß estradiol, groups 4, 5, and 6 received 380mg/kg, 720mg/kg, and 1100mg/kg of Persea americana respectively. These doses of the extract were co-administered with 17ß Estradiol in groups 7, 8, and 9 respectively. Doses were determined from the acute toxicity studies using the Up and Down method. Blood was collected for hormonal analysis and the organs were harvested for organ morphometry and histological analysis after 8 days of administration. The uterine weight of Persea americana + estradiol treated groups significantly increased whereas the body weight decreased compared to group 3. Estrogen and FSH significantly increased in groups 7, 8, and 9 compared to estradiol treated group. Estrogen significantly increased in Persea americana treated groups when compared to group 2. There was evidence of the cuboidal epithelium being stratified, hypertrophy of the endometrial glands, and thickened endometrium in both Persea americana treated groups and Persea americana + estradiol treated groups compared to groups 2 and 3 respectively. It could be concluded that methanol extract of Persea americana is a potential source for estrogen
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References
1. Zhao Y, Zheng H, Xu Y, Lin N. Estrogenic effect of the extract of QingYan formula on reproductive tissues in immature mice. Evid Based Complement Alternat Med. 2019;10:1155.
2. Hall JE. Guyton and Hall textbook of medical physiology. 13th ed. Saunders: Elsevier Inc; 2016.
3. Constanzo LS. Physiology. 5th ed. Saunders: Elsevier Inc; 2014.
4. Fritz R, Jindal S, Feil H, Buyuk E. Elevated serum estradiol levels in artificial autologous frozen embryo transfer cycles negatively impact ongoing pregnancy and live birth rates. J Assist Reprod Genet. 2017;34:133-138.
5. Nees LK, Heublein S, Steinmacher S, Juhasz-Boss I, Brucker S, Tempfer CB, Wallwiener M. Endometrial hyperplasia as a risk factor of endometrial cancer. Arch Gynecol Obstet. 2022;1-15.
6. Ma WG, Song H, Das SK, Paria BC, Dey S. Estrogen is a critical determinant that specifies the duration of the window of uterine receptivity for implantation. Proc Natl Acad Sci U S A. 2003;100:2963-2668.
7. Silvestris E, Pergola G, Rosania R, Loverro G. Obesity as disruptor of the female fertility. Reprod Biol Endocrinol. 2018;16:22.
8. Segovia FJ, Hidalgo GI, Villansante J, Ramis X, Almajano MP. Avocado seed: A comparative study of antioxidant content and capacity in protecting oil models from oxidation. Molecules. 2018;23(10):2421.
9. Orabueze O, Okoko I, Asare G. Evaluation of possible effects of Persea americana seeds on female reproductive hormonal and toxicity profile. J Ethnopharmacol. 2021;273:113870.
10. Araújo RG, Rodriguez-Jasso RM, Ruiz HA, Pintado ME, Noé C. Avocado by-products: Nutritional and functional properties. Trends Food Sci Technol. 2018;80:51-60.
11. Mahadeva R. Phytochemical screening and in-vitro antioxidant and antidiabetic potentials of Persea americana Mill. (Lauraceae) fruit extract. Univ J Pharm Res. 2018;3(5):38-45.
12. Krishna GS, Kumar PP, Ramesh BT. Fruits and vegetables that resemble body organs and have significant role on them. Am J Phytomed Clin Ther. 2014;2(3):277-285
13. National Institute of Health. Infertility (fact sheet). 2020 [cited 2025 Feb 21]. Available from: https://www.nichd.nih.gov/health/topics/factsheets/infertility.
14. Lasota A., Danowska-Klonowska, D. Experimental osteoporosis-different methods of ovariectomy in female white rats. Rocz Akad Med Bialymst. 2004;49(1):129 - 131.
15. Ahuja B. R. Analgesic Effect of Intrathechal Ketamine In Rats. BJA: Brit J Anaesthes (1983); 55(10):991 - 995.
16. El-Ishaq A, Alshawsh MA, Mun KS, Chik Z. Phytochemical screening and anti-implantation activity of Asparagus africanus root extract in female Sprague-Dawley rats. Rev Bras Farmacogn. 2019;29:621-630.
17. Jimenez P, Garcia P, Quitral V, Vasquez K, Parra-Ruiz C, Reyes-Farias M, Diaz DF, Robert P, Encina C, Soto-Covasich J. Pulp, leaf, peel and seed of avocado fruit: A review of bioactive compounds and healthy benefits. Food Rev Int. 2021;619-655.
18. Freitas MS, Pereira AH, Pereira GO, Menezes IS, Lucena AR, Almeida CR, Pereira EG, Santos LA, Tozin LRS, Alves FM, Macedo AL, Silva DB, Ubiali DG. Acetogenin-induced fibrotic heart disease from avocado (Persea americana, Lauraceae) poisoning in horses. Toxicon. 2022;219:10692119.
19. Aguirre LS, Sandovala GV, Medina DM, Martinez OG, Micheloud JF. Acute heart failure in rabbits by avocado leaf poisoning. Toxicon. 2019;164:16-19.
20. Diwan FH, Abdel-Hassan IA, Mohammed ST. Effect of saponin on mortality and histopathological changes in mice. East Mediterr Health J. 2000;6(2):345-351.
21. 21.Melgara B, Diasa MI, Ciricc A, Sokovicc M, Castellob GM, Rodriguez-Lopezd AD, Barrosa L, Ferreiraa IC. Bioactive characterization of Persea americana Mill. by-products: A rich source of inherent antioxidants. Ind Crops Prod. 2018;111:212-218.
22. Baulland DNA, Narcisse VB, Herve T, Adamou M, Momo CMM, Nadege DM, Adam MTM, Ferdinand N. Influence of ethanolic extract of avocado (Persea americana Mill) seed flour on the estrous cycle, the serum concentrations of reproductive hormones, and the activities of oxidative stress markers in female cavies (Cavia porcellus L.). J Adv Vet Anim Res. 2021;8(3):501-510.
23. Orabueze O, Okoko I, Asare G. Evaluation of possible effects of Persea americana seeds on female reproductive hormonal and toxicity profile. J Ethnopharmacol. 2021;273:113870.
24. Sergio RP, Susana RM, Alberto DJ, Socorro RM. Leucaena leucocephala extract has estrogenic and antiestrogenic actions on female rat reproduction. Physiol Behav. 2019;211:112683.
25. Tatsinkou AS, Miegoue E, Tendonkeng F, Mube H, Noumbissi MNB, Mouchili M, Fossi J, Pamo ET. Effect of aqueous and hydroethanolic extracts of avocado seeds (Persea americana) on nutrient digestibility in guinea pigs (Cavia porcellus). Anim Husb Dairy Vet Sci. 2020;4:1-5
26. Qiu S, Vazquez JT, Boulger E, Liu H, Xue P, Hussain MA, Wolfe A. Hepatic estrogen receptor α is critical for regulation of gluconeogenesis and lipid metabolism in males. Sci Rep. 2017;7:1661.
27. Padmanabhan M, Arumugam G. Effect of Persea americana (avocado) fruit extract on the level of expression of adiponectin and PPAR-γ in rats subjected to experimental hyperlipidemia and obesity. J Complement Integr Med. 2014;11(2):107-19.
28. DiNicolantonio JJ, O'Keefe JH. Monounsaturated fat vs saturated fat: Effects on cardio-metabolic health and obesity. Mo Med. 2022;119(1):69-73.
29. Brai BIC, Odetola AA, Agomo PU. Effects of Persea americana leaf extracts on body weight and liver lipids in rats fed hyperlipidaemic diet. Afr J Biotechnol. 2007;6(8):1007-1011.
30. Fida R, Wahab S, Naz A, Hira NE, Khan A, Publishing G. Effect of tocopherol supplementation on endometrial thickness in subfertility patients. Int J Health Sci. 2023; 6(57): 7089-7096
31. Salleh N, Helmy MM, Fadila KN, Yeong SO. Isoflavone genistein induces fluid secretion and morphological changes in the uteri of post-pubertal rats. Int J Med Sci. 2013;10(6):665-675
32. Gueyo TN, Mvondo MA, Zingue S, Sipping MTK, Kenmogne LV, Ndinteh DT, Njamen D. Anthonotha macrophylla P. Beauv (Caesalpiniaceae) aqueous extract exhibits antiestrogenic effects in vitro and in vivo. J Basic Clin Physiol Pharmacol. 2020;31
33. Essono SM, Mvondo MA, Ngadjui E, Nguimatio FXK, Watcho P. The ethanol extract of avocado (Persea americana Mill. (Lauraceae)) seeds successfully induces implant regression and restores ovarian dynamic in a rat model of endometriosis. Evid Based Complement Alternat Med. 2020;20(10):1-10.
34. National Research Council. Guide for the care and use of laboratory animals. 8th ed. Washington, DC: National Academies Press; 2011
35. Ogundele SO, Ajose FO, Kuyinu YA, Odusanya OO. Ethics and governance of clinical research in a developing country: A cross-sectional survey among resident physicians in Nigeria. Pharm Med. 2014;28:249-254.