Regulation of the Cell Cycle and Induction of Apoptosis in 4T1 Breast Cancer Cells by Phenolic Compound from Sterculia quadrifida Bark

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Published: Apr 30, 2025
DOI: https://doi.org/10.26538/tjnpr/v9i4.45
Keywords:
4T1 cell line, Phenolic, Cell cycle, Apoptosis

Main Article Content

Rollando Rollando
Pharmacy Department, Faculty of Health Sciences, Ma Chung University, Malang 65151, Indonesia
Eva Monica
Pharmacy Department, Faculty of Health Sciences, Ma Chung University, Malang 65151, Indonesia
Haryanto F.X. Susanto
Pharmacy Department, Faculty of Health Sciences, Ma Chung University, Malang 65151, Indonesia
Ellyvina Setya Dhini
Pharmacy Department, Faculty of Health Sciences, Ma Chung University, Malang 65151, Indonesia
Annisa Lazuardy
Pharmacy Department, Faculty of Health Sciences, Ma Chung University, Malang 65151, Indonesia
Viol Dhea Kharisma
Doctoral Student of Biology, Faculty of Science and Technology, Universitas Airlangga, Surabaya
Dodi Iskandar
Polytechnic State Pontianak, Pontianak, West Kalimantan
Arif N. M. Ansori
Postgraduate School, Universitas Airlangga, Surabaya, Indonesia

Abstract

Breast cancer remains a significant global health concern, necessitating the ongoing search for more effective treatment strategies. Natural phenolic compounds have gained attention for their anticancer properties among potential therapeutic agents. This study investigates the cytotoxic effects of a phenolic compound on 4T1 breast cancer cells, particularly its role in inducing cell death and disrupting the cell cycle. 4T1 cells were exposed to cisplatin (3.56 µg/mL) and a phenolic compound (5.46 µg/mL), followed by flow cytometry analysis of apoptosis and cell cycle distribution. The results demonstrated that phenolic treatment induced the highest level of cell death (10.13%), exceeding that of cisplatin (6.61%). Additionally, phenolic compounds significantly promoted apoptosis and caused cell cycle arrest, accumulating cells in the S phase. These findings suggest that phenolic compounds may serve as more potent anticancer agents than cisplatin, primarily through apoptosis induction and cell cycle regulation. Further research is needed to elucidate their molecular mechanisms and explore their potential in breast cancer therapy.

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How to Cite
Rollando, R., Monica, E., Susanto, H. F., Dhini, E. S., Lazuardy, A., Kharisma, V. D., Iskandar, D., & Ansori, A. N. M. (2025). Regulation of the Cell Cycle and Induction of Apoptosis in 4T1 Breast Cancer Cells by Phenolic Compound from Sterculia quadrifida Bark. Tropical Journal of Natural Product Research (TJNPR), 9(4), 1700 – 1705. https://doi.org/10.26538/tjnpr/v9i4.45
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Vol. 9 No. 4 (2025): Tropical Journal of Natural Product Research (TJNPR)
Section
Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Author Biographies

Rollando Rollando, Pharmacy Department, Faculty of Health Sciences, Ma Chung University, Malang 65151, Indonesia

Drug Discovery and Design Group Research, Faculty of Health Sciences, Ma Chung University, Malang 65151, Indonesia

Eva Monica, Pharmacy Department, Faculty of Health Sciences, Ma Chung University, Malang 65151, Indonesia

Drug Discovery and Design Group Research, Faculty of Health Sciences, Ma Chung University, Malang 65151, Indonesia

Haryanto F.X. Susanto, Pharmacy Department, Faculty of Health Sciences, Ma Chung University, Malang 65151, Indonesia

Drug Discovery and Design Group Research, Faculty of Health Sciences, Ma Chung University, Malang 65151, Indonesia

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