Tridax Procumbens Leaf Ameliorates Crude Oil-Contaminated Water-Induced Erectile Dysfunction by Mopping Cavernosa Free Radicals and Upregulating Phosphodiesterase 5 and Testosterone
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Abstract
Studies have examined the ameliorative effect of Tridax procumbens leaf (TPL) on erectile dysfunction of several etiologies. However, the role of TPL on crude oil-contaminated water source-induced (CW) erectile dysfunction and the role of phosphodiesterase 5 have not been investigated. This study explores the effect of TPL treatment on CW-induced cavernosa tissue dysfunctions. Twenty adult male Wistar rats were segregated into four groups and treated with distilled water, CW (2.5 ml), CW (2.5 ml) + TPL (100 mg/kg), and TPL only (100 mg/kg), respectively, by oral gavage for five weeks. Cavernosa phosphodiesterase 5, contractile functions, oxidative biomarkers, and serum testosterone were estimated. Body weight, testosterone, cavernosa tissue catalase, superoxide dismutase, and phosphodiesterase 5 were significantly reduced in the CW-only group. The malondialdehyde concentration increased significantly in the CW-only group. The relaxation response (%) of the cavernosa tissue to cumulative doses of acetylcholine after precontraction in phenylephrine was increased considerably in the TPL-only treated group. The group treated with CW-only showed a significant increase (62.2%) in relaxation (%) with incubation in sodium nitroprusside when compared to the control (48.9%), CW+TPL (40.4%), and TPL (46.6%) groups. Acetylcholine-mediated relaxation was significantly increased after incubation with nifedipine in the CW+ TPL (40.6%) group compared to the CW-only (18.3%) treated group. The contraction to the cumulative dose of phenylephrine was significantly inhibited in the TPL-treated groups. Potassium chloride-induced contraction was significantly inhibited in the TPL group. TPL reduces crude oil-contaminated water-induced erectile dysfunctions by mopping cavernosa free radicals, elevating cavernosa phosphodiesterase 5, and serum testosterone.
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