GC–MS Analysis of Bioactive Compounds of Ethanol Extract of Soybean Glycine Max (L.) Merril.
Main Article Content
Abstract
The plant species Glycine max (L.) Merril is commonly called soybean and originates from the Fabaceae family. Soybeans have been cultivated by humans in mainland China since 2500 BC and then spread to other countries along with the increase in trade between countries in the early 19th century. For thousands of years, soybeans have been used as food and medicine in China, Japan, and Korea. The study aimed to identify bioactive compounds in the ethanol extract of Glycine max (L.) Merril soybean. GC-MS Ultra QP 2010 (Shimadzu) was used to identify bioactive compounds in the ethanol extract of Glycine max (L.) Merril soybean. GC-MS showed that the soybeans contained 50 compounds. Retention time, molecular formula, molecular weight, peak area, structure, compound category, and activity were identified. The most abundant compounds found were Isopropyl linoleate (55.25%), n-hexadecanoic acid (24.77%), 6-octadecenoic acid, methyl ester, (Z)- (10.72%), hexadecanoic acid, methyl ester (4.03%) and Methyl stearate (1%). This study shows that the species Glycine max (L.) Merril contains bioactive compounds, including fatty acids, esters, terpenoids, flavonoids, steroids, etc. The presence of these phytoconstituents may justify its traditional use as a source of food and medicine.
Downloads
Article Details

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
1. Srivastava JN, Singh AK. Prevention and Management of Plant Diseases through Plant Products. In: Diseases of Horticultural Crops [Internet]. Boca Raton: Apple Academic Press; 2022. p. 251–268.
2. Sharma P, Manchanda R, Goswami R, Chawla S. Biodiversity and Therapeutic Potential of Medicinal Plants. In: Environmental Concerns and Sustainable Development. Singapore: Springer Singapore; 2020. p. 27–44.
3. Nunes C dos R, Barreto Arantes M, Menezes de Faria Pereira S, Leandro da Cruz L, de Souza Passos M, Pereira de Moraes L, Curvicino Vieira IJ, Barros de Oliveira D. Plants as Sources of Anti-Inflammatory Agents. Molecules. 2020;25(16):3726.
4. Tariq L, Bhat BA, Hamdani SS, Mir RA. Phytochemistry, Pharmacology and Toxicity of Medicinal Plants. In: Medicinal and Aromatic Plants [Internet]. Cham: Springer International Publishing; 2021. p. 217–240.
5. Son YO, Lee JC. Glycoproteins and Polysaccharides are the Main Class of Active Constituents Required for Lymphocyte Stimulation and Antigen-Specific Immune Response Induction by Traditional Medicinal Herbal Plants. J Med Food. 2017; 20(10): 1011-1021.
6. Geszke-Moritz M, Nowak G, Moritz M. Pharmacological Properties and Safe Use of 12 Medicinal Plant Species and Their Bioactive Compounds Affecting the Immune System. Appl Sci. 2023;13(11):6477.
7. Monalisa, Fakih M, Perbawati C. Relevance of WHO traditional medicine strategy (2014-2023) with traditional health care policy in the perspective of national law and international law. Asian J Leg Stud. 2022;1(1):25–34.
8. W.H.O. Traditional Medicine Strategy (2002–2005). WHO/EDM/TRM/2002.1. 2002;
9. Malenčić D, Popović M, Miladinović J. Phenolic Content and Antioxidant Properties of Soybean (Glycine max (L.) Merr.) Seeds. Molecules. 2007;12(3):576–581.
10. Giradkar P, Kulkarni D., More D. Understanding the antioxidant mechanism of Soybean (Glycine max L. Merril). Int J Res Indian Med. 2020;04(4):1–11.
11. Fridayanti A, Purwanto DA, Hendradi E, Preliminary Phytochemical Screening and GC-MS Analysis of Ethanol Extract of Bulbs of Eleutherine sp. Trop J Nat Prod Res. 2022; 6(3):361-364.
12. Otuokere IE, Akoh OU, Echeme JO, Nwadire FC, Nwankwo CI, Egbucha JN, Ammasai, K. GC-MS Analysis and Molecular Docking Studies to Identify Potential SARS-CoV-2 Nonstructural Protein Inhibitors from Icacina trichantha Oliv Tubers. Trop J Nat Prod Res. 2022; 6(8):1336-1342
13 McLafferty, Stauffer. The Wiley/NBS Registry of Mass Spectral Data. New York: J Wiley and Sons; 1989.
14. Stein. Mass Spectral Database and Software. Gaithersburg USA: National Institute of Standards and Technology (NIST); 1990.
15. Vazquez-Roig P, Pico Y. Gas chromatography and mass spectroscopy techniques for the detection of chemical contaminants and residues in foods. In: Chemical Contaminants and Residues in Food. Elsevier; 2012. p. 17–61.
16. Song H, Liu J. GC-O-MS technique and its applications in food flavor analysis. Food Res Int. 2018;114:187–198.
17. Đurović S. Application of the GC/MS technique in environmental analytics: Case of the essential oils. In: Green Sustainable Process for Chemical and Environmental Engineering and Science. Elsevier; 2021. p. 197–208.
18. Feroz S. GC-MS Analysis of Fixed Oils of Nigella Sativa Seeds. Univers J Pharm Res. 2018;3(2):38–42.
19. Rozirwan R, Hananda H, Nugroho RY, Apri R, Khotimah NN,Fauziyah F, Putri WA, Aryawati R. Antioxidant Activity, Total Phenolic, Phytochemical Content, and HPLC Profile of Selected Mangrove Species from Tanjung Api-Api Port Area, South Sumatra, Indonesia. Trop J Nat Prod Res. 2023; 7(7):3482-3489. Doi.org/10.26538/tjnpr/v7i7.29
20. Rajendran P, Bharathidasan R, Sureka. Phytochemical Screening GC-MS and FT-IR Analysis of Sugarcane Juice. Int J Pharma Res Health Sci. 2017;5(6):1962-7.
21. Aftab T, Hakeem KR. Medicinal and Aromatic Plants Healthcare and Industrial Applications [Internet]. Aftab T, Hakeem KR, editors. Cham: Springer International Publishing; 2021.
22. Kumar N, Goel N. Phenolic acids: Natural versatile molecules with promising therapeutic applications. Biotechnol Reports. 2019;24: 1-10.
23. Pinto ME, Araújo SG, Morais MI, Sa NP, Lima CM, Rosa CA, Siqueira EP, Johann S, Lima. Antifungal and antioxidant activity of fatty acid methyl esters from vegetable oils. An Acad Bras Cienc. 2017;89(3):1671–1681.
24. Adnan M, Nazim Uddin Chy M, Mostafa Kamal ATM, Azad MOK, Paul A, Uddin SB, Barlow JW, Faruque MO, Park CH, Cho DH. Investigation of the Biological Activities and Characterization of Bioactive Constituents of Ophiorrhiza rugosa var. prostrata (D.Don) & Mondal Leaves through In Vivo, In Vitro, and In Silico Approaches. Molecules. 2019;24(7):1-24.