Membrane Stabilization, Albumin Denaturation, Protease Inhibition, and Antioxidant Activity as Possible Mechanisms for the Anti-Inflammatory Effects of Flavonoid-Rich Extract of Peltophorum pterocarpum (DC.) K. Heyne (FREPP) Stem Bark 

Osmund C. Enechi1, Emmanuel S. Okeke1,2,3*, Nicodemus E. Nwankwo1,2, John E. Nweze1, Chukwuebuka P. Obilor1, Christopher I. Okoye1, Ogochukwu E. Awoh1

1Department of Biochemistry, University of Nigeria, Nsukka, Enugu State, Nigeria
2Natural Science Unit, School of General Studies, University of Nigeria, Nsukka, Enugu State, Nigeria
3School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, PRC

Corresponding Author: emmanuel.okeke@unn.edu.ng; Tel: +2348035277554
Recieved Date: August 29, 2020; Accepted Date: October 25, 2020; Published Date: 02 November 2020
Citation: Enechi CO, Okeke SE, Nwankwo EN, Nweze JE, Obilor CP, Okoye CI, Awoh OE. Membrane Stabilization, Albumin Denaturation, Protease Inhibition, and Antioxidant Activity as Possible Mechanisms for the Anti-Inflammatory Effects of Flavonoid-Rich Extract of Peltophorum pterocarpum (DC.) K. Heyne (FREPP) Stem Bark. Trop J Nat Prod Res. 2020; 4(10):812-816.   https://doi.org/10.26538/tjnpr/v4i10.25
Copyright: © 2020 Enechi et al This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

The ever increasing discovery of  large number medicinal plants and the   screening of their bioactivity to provide data that will help physicians and patients make wise decision before using them. The study evaluated the membrane stabilization, albumin denaturation, protease inhibition and antioxidant activities as possible mechanisms for the anti-inflammatory effects of the stem-bark flavonoid-rich extract of Peltophorum pterocarpum (FREPP). The antioxidant activities were assessed using the hydrogen peroxide, nitric oxide (NO) and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assays. The anti-inflammatory effect of FREPP was determined using the membrane stabilization model, albumin denaturation, protease inhibitor assay, while the total flavonoid was examined using the aluminium chloride colorimetric test. At 0.1, 0.4, 0.8 mg/mL, FREPP inhibited hypotonicity-induced haemolysis by 61.03, 76.84 and 83.84%, respectively. FREPP was effective in inhibiting albumin denaturation in a concentration-dependent manner with the highest inhibition of 69.89% at 50 µg/mL. Protease activity was significantly (P < 0.05) reduced at all concentrations compared to the control. The results showed that FREPP has antioxidant and anti-inflammatory activities.

Keywords: Peltophorum pterocarpum, Stem bark, Antioxidant, Anti-inflammatory, Flavonoid.
Back to Articles