Alpha-Amylase Inhibitory Activity of Extract Combination of Morinda citrifolia L. Fruit, Trigonella foenum-graecum and Nigella sativa L. Seeds Using In vitro and In Vivo Assay

Musfiroh Ida1*, Muhtadi Ahmad2, Apriliani N. Dwi2
1Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Indonesia
2Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Indonesia

Corresponding Author: ida.musfiroh@unpad.ac.id; Tel: +628116265675
Recieved Date: August 23, 2020; Accepted Date: October 28, 2020; Published Date: 02 November 2020
Citation: Ida M, Ahmad M, Dwi AN. Alpha-amylase Inhibitory Activity of Extract Combination of Morinda citrifolia L. Fruit, Trigonella foenum-graecum and Nigella sativa L. Seeds Using In vitro and In Vivo Assay. Trop J Nat Prod Res. 2020; 4(10):801-805.  https://doi.org/10.26538/tjnpr/v4i10.23
Copyright: © 2020 Ida et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

The poly-herbal formulation is being evaluated for effective diabetes management. However, only a few formulations have made significant inroads as treatment alternatives. This research was carried out to determine the in vitro and in vivo alpha-amylase inhibitory activity of extract combination of Morinda citrifolia L fruits, Trigonella foenum-graecum and Nigella sativa L. seeds, because each extract was known to have antidiabetic properties. In vitro alpha amylase activity was carried out using dinitrosalicylic acid (DNS) assay while the in vivo alpha amylase activity was performed using experimental rat model. Twenty-four mice (Mus musculus L.) were randomly divided into six groups. Group 1 served as the control and was given distilled water only, while group II was induced with alloxan (40 mg/kg i.p.) only. Groups III, IV, V and VI rats were induced with alloxan, then after 48 h of induction, the rats were given acarbose, dose 1 (70:70:280 mg/kg BW): dose 2 (140:70:140 mg/kg BW) and dose 3 (70:140:140 mg/kg BW), respectively. The three combination of extracts showed inhibitory alpha-amylase activity, with combination of dose 2 as the best potential inhibition. The combination of dose 2 has an IC50 value of 35.70 ± 1.33 µg/mL and the percentage reduction in the average blood glucose levels at the 30th, 60th, 120th minute were shown to be 11.98%, 25.85%, and 34.80%, respectively.   

Keywords: Blood glucose level, Diabetes mellitus, IC50, Morinda citrifolia L., Nigella sativa L., Trigonella foenum-graecum L.
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