Qualitative and Quantitative Phytochemicals Studies of Ethanol Stem bark Extracts of Isoberlinia doka Craib & Stapf and Isoberlinia tomentosa (Harms) Craib & Stapf

Bello Hadiza1*, Katsayal A. Umar1, Ambi A. Aminu1, Abubakar Y. Bashir2
1Department of Pharmacognosy and Drug Development, Faculty of Pharmaceutical Sciences, Ahmadu Bello University, Zaria, Nigeria
2Department of Botany, Faculty of Life Science, Ahmadu Bello University, Zaria, Nigeria

Corresponding Author: hbello855@gmail.com; Tel: +2347064795286
Recieved Date: June 25, 2020; Accepted Date: October 30, 2020; Published Date: 02 November 2020
Citation: Hadiza B, Umar KA, Aminu AA, Bashir AY. Qualitative and Quantitative Phytochemicals Studies of Ethanol Stem bark Extracts of Isoberlinia doka Craib & Stapf and Isoberlinia tomentosa (Harms) Craib & Stapf. Trop J Nat Prod Res. 2020; 4(10):756-764.  https://doi.org/10.26538/tjnpr/v4i10.16
Copyright: © 2020 Hadiza et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Isoberlinia doka and Isoberlinia tomentosa are plant used in traditional treatment of various kinds of diseases in Nigeria without scientific validation on the phytochemicals responsible for their actions. The present work was aimed to perform phytochemical analysis on the stem bark crude extracts and fractions of I. doka and I. tomentosa. The qualitative and quantitative phytochemicals studies were conducted on I. doka and I. tomentosa based on standard method. The findings from qualitative phytochemical analysis showed the presence of flavonoids, tannins, terpenoid/steroid, saponins and alkaloids whereas anthraquinones were absent in all the extracts and fractions of I. doka and I. tomentosa. The phytochemicals content of the crude extract and fractions of I. doka were found to be higher than I. tomentosa except for total saponin content. The crude extract of I. doka has the highest total alkaloids content (42.1 mg/g), the highest total flavonoids and phenol contents were obtained from n-Butanol fraction of I. doka (139.1 mg/g and 310.0 mg/g, respectively). However, the highest total saponin content was obtained as 170.6 mg/g in I. tomentosa crude ethanol extract. Therefore I. doka and I. tomentosa were very rich in similar phytochemical which could be responsible for their claimed efficiency in traditional medicine for the prevention of several diseases.

Keywords: UV-VIS Spectroscopy, Phytochemical screening, Leaf extracts, Isoberlinia.
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