Comparative Botanical and Genetic Diversity Study of Different Cultivars of Sesamum indicum L. Cultivated in Egypt

Seham S. El-Hawary1, Rehab M. S. Ashour1, Mahitab H. El-Bishbishy2, Mona M. Okba1*, Heba A. Hassan2*
1Pharmacognosy Department, Faculty of Pharmacy, Cairo University, 11562, Giza, Egypt
2Pharmacognosy Department, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), 11787, Giza, Egypt

Corresponding Author: mona.morad@pharma.cu.edu.eg; hahahmed@msa.eun.eg; Tel: +202 3837-1516
Recieved Date: September 23, 2020; Accepted Date: October 26, 2020; Published Date: 02 November 2020
Citation: El-Hawary SS, Ashour RMS, El-Bishbishy MH, Okba MM, Hassan HA. Comparative Botanical and Genetic Diversity Study of Different Cultivars of Sesamum indicum L. Cultivated in Egypt. Trop J Nat Prod Res. 2020; 4(10):748-755. https://doi.org/10.26538/tjnpr/v4i10.15
Copyright: © 2020 El-Hawaryet al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Sesame (Sesamum indicum L.) is a plant belonging to the Family Pedaliaceae. It grows worldwide in India, Sudan and China. The high nutritive and curative effects of sesame favour its wide use in culinary and traditional medicines in many countries. No reports could be traced concerning the macromorphological and micromorphological characterization or genetic profiling of S. indicum L. cultivars (Shandawel-3, Giza-32 and Toshka) cultivated in Egypt. The establishment of proper identification and authentication of such valuable plant deemed necessary. Therefore, botanical and genetic diversity study of the three cultivars using RAPD markers were investigated. Detailed morphological study through examination of transverse sections in the lamina and midrib region, examination of surface preparations and powders of the three cultivars.Also, Genetic study was performed using twelve primers producing a total of ninety-nine RAPD fragments, of which twenty-five bands (25.25%) were polymorphic. The number of bands per primer ranged from two to fourteen, while the number of polymorphic bands ranged from zero to six and monomorphic bands ranged from two to twelve. Unique bands were observed with eleven primers. Unweighted pair group method using arithmetic average (UPGMA) clustering resulted in two major groups. Results showed that all cultivars have nearly similar macro and micromorphological characters but differ in the dimensions of certain elements with high level of genetic similarity. Thus, this study helped in providing a useful tool for identification of sesame for germplasm banks maintenance and effective parents selection in breeding programs.

Keywords: Genetic diversity, Macromorphology, Micromorphology, RAPD, Sesamum indicum L., UPGMA.
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