Hesperidin and Myricetin Attenuated Non-Alcoholic Fatty Liver Disease (NAFLD) in HepG2 Cells

Nadta Sukkasem1, Waranya Chatuphonprasert2, Kanokwan Jarukamjorn1*
1Research Group for Pharmaceutical Activities of Natural Products using Pharmaceutical Biotechnology (PANPB), Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002 Thailand
2Faculty of Medicine, Mahasarakham University, Maha Sarakham 44000 Thailand

Corresponding Author: kanok_ja@kku.ac.th; Tel: +66-43-202379
Recieved Date: September 23, 2020; Accepted Date: October 24, 2020; Published Date: 02 November 2020
Citation: Sukkasem N, Chatuphonprasert W, Jarukamjorn K. Hesperidin and myricetin attenuated non-alcoholic fatty liver disease (NAFLD) in HepG2 cells. Trop J Nat Prod Res. 2020; 4(10):739-747.  https://doi.org/10.26538/tjnpr/v4i10.14
Copyright: © 2020 Sukkasem et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Flavonoids are natural compounds that might have beneficial effects on non-alcoholic fatty liver disease (NAFLD). This study determined the efficacy of flavonoids in an NAFLD cell model. HepG2 cells were treated for 24 h with either 1 mM oleic acid (OA) or palmitic acid (PA) and 10 ?M of one of eleven flavonoids (4 flavones, 2 flavanones, and 5 flavonols) or fenofibrate. Cell morphology was examined by oil-red-O staining. Metabolic gene expression (PPAR ?/?, SREBP 1a/1c, ACC, ACOX, and FAS) was analyzed by RT/qPCR. All flavonoids and fenofibrate reduced intracellular lipid content. Hesperidin, myricetin, and all tested flavanones and flavonols prevented fatty acid-induced increases in metabolic gene expression but fenofibrate and some flavones did not suppress OA-induced FAS expression. Hesperidin and myricetin suppressed metabolic gene expression by more than fenofibrate indicating that they may provide potential pharmacological treatment options for regression of NAFLD status.

Keywords: Flavonoids, Oleic acid, Palmitic acid, PPARs, SREBPs, Fenofibrate.
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