Novel Phage Cocktail for the Treatment of Bacteria Causing Chronic Suppurative Otitis Media

Sadeq A. G. Kaabi, Hadeel K. Musafer, Saba T. Hashim, Zahraa K. Raheem*
Biology Department, College of Science, Mustansiriyah University, Baghdad, Iraq
Corresponding Author: sadeqkaabi@uomustansiriyah.edu.iq; Tel: 009647709023245
Recieved Date: July 16, 2020; Accepted Date: October 19, 2020; Published Date: 02 November 2020
Citation: Kaabi SAG, Musafer HK, Hashim ST, Raheem ZK. Novel Phage Cocktail for the Treatment of Bacteria Causing Chronic Suppurative Otitis Media. Trop J Nat Prod Res. 2020; 4(10):680-686.  https://doi.org/10.26538/tjnpr/v4i10.4
Copyright: © 2020 Kaabi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT

Chronic suppurative otitis media (CSOM) is a chronic inflammation of the middle ear cavity and mastoid cavity. It occurs in any community in a percentage of 0.5-33% for all age group. The objective of this study was to isolate and prepare phage cocktail active against bacteria causing chronic suppurative otitis media from sewage water. Bacteria were isolated from non-drug responsive cases of CSOM.  A total of 73 ear swabs were collected from patients of CSOM and showed bacterial growth in 63 (86.3%) and fungal growth in 10 (13.7%). The most prevalent bacterium was Pseudomonas aeruginosa which was found in 33 swabs (45.2%), followed by Staphylococcus aureus in 15 (20.5%). Species of Klebsiella pneumoniae, Escherichia coli, Proteus mirabilis, and Moraxella catarrhalis were isolated in 5 (6.8%), 5 (6.8%), 4 (5.5%) and 1 (1.3%), respectively. The most potent phages were 7, 3, 1, 2, 1 and 1 for species of P. aeruginosa, S. aureus, K. pneumoniae, E. coli, P. mirabilis and M. catarrhalis, respectively. Phages were isolated after enrichment of each CSOM bacterial pathogen with sewage water by spot lysis method and characterized morphologically by top agarose plaque assay.  Most virulent isolated phages were selected for formulation of phage cocktail against isolated bacterial pathogens of CSOM. A number of 17 phages cocktail were formulated and showed 60-100% inhibition on all tested isolates of P. aeruginosa, S. aureus, K. pneumoniae, E. coli, P. mirabilis and M. catarrhalis in a growth inhibition assay.

Keywords: Chronic suppurative otitis media, Phage therapy, Drug resistance, Phage cocktail.
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